Atomic force microscopy measurements reveal multiple bonds between Helicobacter pylori blood group antigen binding adhesin and Lewis b ligand
2014 (English)In: Journal of the Royal Society Interface, ISSN 1742-5662, E-ISSN 1742-5689, Vol. 11, no 101, UNSP 20141040- p.Article in journal (Refereed) Published
The strength of binding between the Helicobacter pylori blood group antigen-binding adhesin (BabA) and its cognate glycan receptor, the Lewis b blood group antigen (Leb), was measured by means of atomic force microscopy. High-resolution measurements of rupture forces between single receptor-ligand pairs were performed between the purified BabA and immobilized Leb structures on self-assembled monolayers. Dynamic force spectroscopy revealed two similar but statistically different bond populations. These findings suggest that the BabA may form different adhesive attachments to the gastric mucosa in ways that enhance the efficiency and stability of bacterial adhesion.
Place, publisher, year, edition, pages
The Royal Society , 2014. Vol. 11, no 101, UNSP 20141040- p.
Helicobacter pylori, blood group antigen-binding adhesin, Lewis b, self-assembled monolayers, adhesion force, atomic force microscopy
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
IdentifiersURN: urn:nbn:se:umu:diva-96487DOI: 10.1098/rsif.2014.1040ISI: 000343672800028OAI: oai:DiVA.org:umu-96487DiVA: diva2:767551