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Metabolomic analysis of extreme freezing tolerance in Siberian spruce (Picea obovata)
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Department of Biology, Norwegian University of Science and Technology.
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2014 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 204, no 3, 545-555 p.Article in journal (Refereed) Published
Abstract [en]

Siberian spruce (Picea obovata) is one of several boreal conifer species that can survive at extremely low temperatures (ELTs). When fully acclimated, its tissues can survive immersion in liquid nitrogen. Relatively little is known about the biochemical and biophysical strategies of ELT survival. We profiled needle metabolites using gas chromatography coupled with mass spectrometry (GC-MS) to explore the metabolic changes that occur during cold acclimation caused by natural temperature fluctuations. In total, 223 metabolites accumulated and 52 were depleted in fully acclimated needles compared with pre-acclimation needles. The metabolite profiles were found to develop in four distinct phases, which are referred to as pre-acclimation, early acclimation, late acclimation and fully acclimated. Metabolite changes associated with carbohydrate and lipid metabolism were observed, including changes associated with increased raffinose family oligosaccharide synthesis and accumulation, accumulation of sugar acids and sugar alcohols, desaturation of fatty acids, and accumulation of digalactosylglycerol. We also observed the accumulation of protein and nonprotein amino acids and polyamines that may act as compatible solutes or cryoprotectants. These results provide new insight into the mechanisms of freezing tolerance development at the metabolite level and highlight their importance in rapid acclimation to ELT in P.obovata.

Place, publisher, year, edition, pages
2014. Vol. 204, no 3, 545-555 p.
Keyword [en]
acclimation, cold, conifer, frost, GC-MS, metabolites, needles, Siberian spruce (Picea obovata)
National Category
URN: urn:nbn:se:umu:diva-96810DOI: 10.1111/nph.12950ISI: 000343869000014OAI: diva2:770167

Originally included in thesis in manuscript form.

Available from: 2014-12-10 Created: 2014-12-03 Last updated: 2014-12-10Bibliographically approved
In thesis
1. Proteomics and metabolomics in biological and medical applications
Open this publication in new window or tab >>Proteomics and metabolomics in biological and medical applications
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Biological processes in living organisms consist of a vast number of different molecular networks and interactions, which are complex and often hidden from our understanding. This work is focused on recovery of such details for two quite distant examples: acclimation to extreme freezing tolerance in Siberian spruce (Picea obovata) and detection of proteins associated with prostate cancer.

The first biological system in the study, upon P. obovata, is interesting by this species ability to adapt and sustain extremely low temperatures, such as -60⁰C or below. Despite decades of investigations, the essential features and mechanisms of the amazing ability of this species still remains unclear. To enhance knowledge about extreme freezing tolerance, the metabolome and proteome of P. obovata’s needles were collected during the tree’s acclimation period, ranging from mid August to January, and have been analyzed.

The second system within this study is the plasma proteome analysis of high risk prostate cancer (PCa) patients, with and without bone metastases. PCa is one of the most common cancers among Swedish men, which can abruptly develop into an aggressive, lethal disease. The diagnostic tools, including PSA-tests, are insufficient in predicting the disease’s aggressiveness and novel prognostic markers are urgently required.

Both biological systems have been analyzed following similar steps: by two-dimensional difference gel electrophoresis (2D-DIGE) techniques, followed by protein identification using mass spectrometry (MS) analysis and multivariate methods. Data processing has been utilized for searching for proteins that serve as unique indicators for characterizing the status of the systems. In addition, the gas chromatography-mass spectrometry (GC-MS) study of the metabolic content of P.obovata’s needles, from the extended observation period, has been performed. The studies of both systems, combined with thorough statistical analysis of experimental outcomes, have resulted in novel insights and features for both P. obovata and prostate cancer. In particular, it has been shown that dehydrins, Hsp70s, AAA+ ATPases, lipocalin and several proteins involved in cellular metabolism etc., can be uniquely associated with acclimation to extreme freezing in conifers. Metabolomic analysis of P. obovata needles has revealed systematic metabolic changes in carbohydrate and lipid metabolism. Substantial increase of raffinose, accumulation of desaturated fatty acids, sugar acids, sugar alcohols, amino acids and polyamines that may act as compatible solutes or cryoprotectants have all been observed during the acclimation process.

Relevant proteins for prostate cancer progression and aggressiveness have been identified in the plasma proteome study, for patients with and without bone metastasis. Proteins associated with lipid transport, coagulation, inflammation and immune response have been found among them. 

Place, publisher, year, edition, pages
Umeå: Kemiska institutionen, Umeå Universitet, 2011. 44 p.
2D-DIGE, biomarkers, cold-acclimation, conifer, freezing tolerance, GC-MS, metabolomics, multiple hypothesis test, multivariate analysis, OPLS-DA, Picea obovata, plasma, prostate cancer, proteomics, ProteoMiner, Siberian spruce
National Category
Chemical Sciences
urn:nbn:se:umu:diva-43520 (URN)978-91-7459-204-7 (ISBN)
Public defence
2011-05-25, KBC-huset, KB3A9, Umeå Universitet, Umeå, 10:00 (English)
Available from: 2011-05-04 Created: 2011-05-03 Last updated: 2014-12-10Bibliographically approved

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Angelcheva, LiudmilaMishra, YogeshAntti, HenrikFunk, ChristianeSchröder, Wolfgang P.
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