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IL-34 Expression in Gingival Fibroblasts, Gingival Crevicular Fluid and Gingival Tissue
Umeå University, Faculty of Medicine, Department of Odontology, School of Dentistry.
2014 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

IL-34 is a protein associated with bone degenerative diseases but the role in periodontal disease is unknown. The aim of this study was to assess the expression of IL-34 in primary human gingival fibroblasts (GF) and investigate if the expression is regulated by the pro-inflammatory cytokines interleukin-1 (IL-1β) and tumor necrosis factor α(TNF-α). We also investigated if IL-34 is detectible in gingival crevicular fluid (GCF) in healthy, gingivitis and periodontitis sites. Furthermore, we examined if healthy and inflamed gingival tissue contains IL-34.

GF were stimulated by IL-1β300 pg/ml and TNF-α10 ng/ml. IL-34 mRNA was measured by quantitative real-time PCR (qPCR). GCF was collected from 11 healthy, 10 gingivitis, and 21 periodontitis gingival crevices. IL-34 protein was quantified using enzyme-linked immunoabsorbent assays (ELISA). Healthy and inflamed gingival tissue biopsies were collected and examined using immunohistochemistry (IHC).

IL-34 mRNA was expressed in GF and the expression was enhanced 12x fold-change versus control by TNF-α10 ng/ml and 4x fold-change versus control by IL-1β300 pg/ml. IL-34 was also present in GCF but no significant difference in IL-34 protein was detected between the healthy, gingivitis, and periodontitis groups. Healthy and inflamed gingival tissue showed equal amounts of IL-34 protein in the epithelium while sub-epithelially the inflamed tissue showed higher levels of IL-34 protein.

Pro-inflammatory cytokines stimulate IL-34 mRNA expression in GF. IL-34 protein is present in GCF and gingival tissue which demands further investigation about the eventual role of IL-34 in the pathogenesis of periodontitis.

Place, publisher, year, edition, pages
National Category
Medical and Health Sciences
URN: urn:nbn:se:umu:diva-97861OAI: diva2:777180
Educational program
Dentistry Programme
Available from: 2015-03-11 Created: 2015-01-08 Last updated: 2015-03-11Bibliographically approved

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