Generic structures of cytotoxic liprotides: nano-sized complexes with oleic acid cores and shells of disordered proteins
2014 (English)In: ChemBioChem (Print), ISSN 1439-4227, E-ISSN 1439-7633, Vol. 15, no 18, 2693-2702 p.Article in journal (Refereed) Published
The cytotoxic complex formed between alpha-lactalbumin and oleic acid (OA) has inspired many studies on protein-fatty acid complexes, but structural insight remains sparse. After having used small-angle X-ray scattering (SAXS) to obtain structural information, we present a new, generic structural model of cytotoxic protein-oleic acid complexes, which we have termed liprotides (lipids and partially denatured proteins). Twelve liprotides formed from seven structurally unrelated proteins and prepared by different procedures all displayed core-shell structures, each with a micellar OA core and a shell consisting of flexible, partially unfolded protein, which stabilizes the OA micelle. The common structure explains similar effects exerted on cells by different liprotides and is consistent with a cargo off-loading of the OA into cell membranes.
Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2014. Vol. 15, no 18, 2693-2702 p.
lipids, micelles, protein folding, protein structures, protein-protein interactions, BYE P, 1947, JOURNAL OF PHYSICAL AND COLLOID CHEMISTRY, V51, P18 ensson M, 1999, JOURNAL OF BIOLOGICAL CHEMISTRY, V274, P6388
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Pharmaceutical Sciences
IdentifiersURN: urn:nbn:se:umu:diva-97883DOI: 10.1002/cbic.201402407ISI: 000346023500010OAI: oai:DiVA.org:umu-97883DiVA: diva2:781361