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High lipid order of Arabidopsis cell-plate membranes mediated by sterol and DYNAMIN-RELATED PROTEIN1A function
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, USA.
Department of Developmental Genetics, Centre for Plant Molecular Biology, University of Tübingen, Tübingen, Germany.
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2014 (English)In: The Plant Journal, ISSN 0960-7412, E-ISSN 1365-313X, Vol. 80, no 5, 745-757 p.Article in journal (Refereed) Published
Abstract [en]

Membranes of eukaryotic cells contain high lipid-order sterol-rich domains that are thought to mediate temporal and spatial organization of cellular processes. Sterols are crucial for execution of cytokinesis, the last stage of cell division, in diverse eukaryotes. The cell plate of higher-plant cells is the membrane structure that separates daughter cells during somatic cytokinesis. Cell-plate formation in Arabidopsis relies on sterol- and DYNAMIN-RELATED PROTEIN1A (DRP1A)-dependent endocytosis. However, functional relationships between lipid membrane order or lipid packing and endocytic machinery components during eukaryotic cytokinesis have not been elucidated. Using ratiometric live imaging of lipid order-sensitive fluorescent probes, we show that the cell plate of Arabidopsis thaliana represents a dynamic, high lipid-order membrane domain. The cell-plate lipid order was found to be sensitive to pharmacological and genetic alterations of sterol composition. Sterols co-localize with DRP1A at the cell plate, and DRP1A accumulates in detergent-resistant membrane fractions. Modifications of sterol concentration or composition reduce cell-plate membrane order and affect DRP1A localization. Strikingly, DRP1A function itself is essential for high lipid order at the cell plate. Our findings provide evidence that the cell plate represents a high lipid-order domain, and pave the way to explore potential feedback between lipid order and function of dynamin-related proteins during cytokinesis.

Place, publisher, year, edition, pages
2014. Vol. 80, no 5, 745-757 p.
Keyword [en]
membrane order, sterol, cytokinesis, DRP1A, Arabidopsis
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URN: urn:nbn:se:umu:diva-98426DOI: 10.1111/tpj.12674ISI: 000345511500001OAI: diva2:786005
Swedish Research Council Formas, 2007-715
Available from: 2015-02-04 Created: 2015-01-22 Last updated: 2015-05-07Bibliographically approved

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Frescatada-Rosa, MarciaStanislas, ThomasMen, ShuzhenBoutte, YohannGrebe, Markus
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Department of Plant PhysiologyUmeå Plant Science Centre (UPSC)
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