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RpoS and Indole Signaling Control the Virulence of Vibrio anguillarum towards Gnotobiotic Sea Bass (Dicentrarchus labrax) Larvae
Ghent, Belgium.
Gent, Belgium.
Ghent, Belgium.
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Southern Research Institute, Birmingham, Alabama, United States of America. (Debra Milton)
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2014 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 9, no 10, e111801- p.Article in journal (Refereed) Published
Abstract [en]

Quorum sensing, bacterial cell-to-cell communication with small signal molecules, controls the virulence of many pathogens. In contrast to other vibrios, neither the VanI/VanR acylhomoserine lactone quorum sensing system, nor the three-channel quorum sensing system affects virulence of the economically important aquatic pathogen Vibrio anguillarum. Indole is another molecule that recently gained attention as a putative signal molecule. The data presented in this study indicate that indole signaling and the alternative sigma factor RpoS have a significant impact on the virulence of V. anguillarum. Deletion of rpoS resulted in increased expression of the indole biosynthesis gene tnaA and in increased production of indole. Both rpoS deletion and the addition of exogenous indole (50-100 mu M) resulted in decreased biofilm formation, exopolysaccharide production (a phenotype that is required for pathogenicity) and expression of the exopolysaccharide synthesis gene wbfD. Further, indole inhibitors increased the virulence of the rpoS deletion mutant, suggesting that indole acts downstream of RpoS. Finally, in addition to the phenotypes found to be affected by indole, the rpoS deletion mutant also showed increased motility and decreased sensitivity to oxidative stress.

Place, publisher, year, edition, pages
PLOS one , 2014. Vol. 9, no 10, e111801- p.
National Category
Cell and Molecular Biology
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URN: urn:nbn:se:umu:diva-96950DOI: 10.1371/journal.pone.0111801ISI: 000343943700152OAI: oai:DiVA.org:umu-96950DiVA: diva2:790553
Available from: 2015-02-25 Created: 2014-12-05 Last updated: 2017-12-04Bibliographically approved

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Milton, Debra L.

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