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Efficient quantification and characterization of bacterial outer membrane derived nano-particles with flow cytometric analysis
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2014 (English)In: International Journal of Medical Microbiology, ISSN 1438-4221, E-ISSN 1618-0607, Vol. 304, no 8, 1032-1037 p.Article in journal (Refereed) Published
Abstract [en]

There currently exists no efficient and easy method for size profiling and counting of membranous nano-scale particles, such as bacterial outer membrane vesicles (OMVs). We present here a cost-effective and fast method capable of profiling and counting small sample volumes of nano-scale membranous vesicles with standard laboratory equipment without the need for any washing steps. OMV populations of different bacterial species are compared and even subpopulations of OMVs can be identified after a simple labelling procedure. Counting is possible over three orders of magnitude without any changes to the protocol. Protein contaminations do not alter the described measurements.

Place, publisher, year, edition, pages
2014. Vol. 304, no 8, 1032-1037 p.
Keyword [en]
Outer membrane vesicles, OMV, Flow cytometry, OMV enumeration, OMV quantification, Escherichia coil
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-99390DOI: 10.1016/j.ijmm.2014.07.012ISI: 000347023400013OAI: oai:DiVA.org:umu-99390DiVA: diva2:791025
Available from: 2015-02-26 Created: 2015-02-07 Last updated: 2017-12-04Bibliographically approved

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Wai, Sun Nyunt

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Department of Molecular Biology (Faculty of Medicine)Molecular Infection Medicine Sweden (MIMS)
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Microbiology in the medical area

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