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The new frontier of genome engineering with CRISPR-Cas9
Univ Calif Berkeley, Berkeley, CA 94720 USA.
Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR). Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Helmholtz Ctr Infect Res, Dept Regulat Infect Biol, D-38124 Braunschweig, Germany; Hannover Med Sch, D-30625 Hannover, Germany.
2014 (English)In: Science, ISSN 0036-8075, E-ISSN 1095-9203, Vol. 346, no 6213, 1077-+ p.Article, review/survey (Refereed) Published
Abstract [en]

The advent of facile genome engineering using the bacterial RNA-guided CRISPR-Cas9 system in animals and plants is transforming biology. We review the history of CRISPR (clustered regularly interspaced palindromic repeat) biology from its initial discovery through the elucidation of the CRISPR-Cas9 enzyme mechanism, which has set the stage for remarkable developments using this technology to modify, regulate, or mark genomic loci in a wide variety of cells and organisms from all three domains of life. These results highlight a new era in which genomic manipulation is no longer a bottleneck to experiments, paving the way toward fundamental discoveries in biology, with applications in all branches of biotechnology, as well as strategies for human therapeutics.

Place, publisher, year, edition, pages
2014. Vol. 346, no 6213, 1077-+ p.
National Category
Biological Sciences Cell and Molecular Biology
URN: urn:nbn:se:umu:diva-98861DOI: 10.1126/science.1258096ISI: 000345763400031OAI: diva2:791712
Available from: 2015-03-02 Created: 2015-01-27 Last updated: 2015-03-02Bibliographically approved

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Charpentier, Emmanuelle
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Molecular Infection Medicine Sweden (MIMS)Umeå Centre for Microbial Research (UCMR)Department of Molecular Biology (Faculty of Medicine)
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