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A multivariate approach to correlate bacterial surface properties to biofilm formation by lipopolysaccharide mutants of Pseudomonas aeruginosa
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Physics.
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2015 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 127, no 0, p. 182-191Article in journal (Refereed) Published
Abstract [en]

Abstract Bacterial biofilms are involved in various medical infections and for this reason it is of great importance to better understand the process of biofilm formation in order to eradicate or mitigate it. It is a very complex process and a large range of variables have been suggested to influence biofilm formation. However, their internal importance is still not well understood. In the present study, a range of surface properties of Pseudomonas aeruginosa lipopolysaccharide mutants were studied in relation to biofilm formation measured in different kinds of multi-well plates and growth conditions in order to better understand the complexity of biofilm formation. Multivariate analysis was used to simultaneously evaluate the role of a range of physiochemical parameters under different conditions. Our results suggest the presence of serum inhibited biofilm formation due to changes in twitching motility. From the multivariate analysis it was observed that the most important parameters, positively correlated to biofilm formation on two types of plates, were high hydrophobicity, near neutral zeta potential and motility. Negative correlation was observed with cell aggregation, as well as formation of outer membrane vesicles and exopolysaccharides. This work shows that the complexity of biofilm formation can be better understood using a multivariate approach that can interpret and rank the importance of different factors being present simultaneously under several different environmental conditions, enabling a better understanding of this complex process.

Place, publisher, year, edition, pages
Elsevier, 2015. Vol. 127, no 0, p. 182-191
Keyword [en]
Pseudomonas aeruginosa, Biofilm, LPS mutant, Multivariate analysis, Bacterial surface properties
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:umu:diva-100362DOI: 10.1016/j.colsurfb.2015.01.030ISI: 000353001400024PubMedID: 25679490OAI: oai:DiVA.org:umu-100362DiVA, id: diva2:791756
Available from: 2015-03-02 Created: 2015-03-02 Last updated: 2018-02-14Bibliographically approved

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Antti, HenrikRzhepishevska, OlenaBoulanger, NicolasBarbero, David R.Wai, Sun NyuntUhlin, Bernt EricRamstedt, Madeleine
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Department of ChemistryDepartment of PhysicsMolecular Infection Medicine Sweden (MIMS)Umeå Centre for Microbial Research (UCMR)
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Colloids and Surfaces B: Biointerfaces
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