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Evidence that processing of ribonucleotides in DNA by topoisomerase 1 is leading-strand specific
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics. (Andrei Chabes)
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2015 (English)In: Nature Structural & Molecular Biology, ISSN 1545-9993, E-ISSN 1545-9985, Vol. 22, no 4, 291-U35 p.Article in journal (Refereed) Published
Abstract [en]

Ribonucleotides incorporated during DNA replication are removed by RNase H2-dependent ribonucleotide excision repair (RER). In RER-defective yeast, topoisomerase 1 (Top1) incises DNA at unrepaired ribonucleotides, initiating their removal, but this is accompanied by RNA-DNA-damage phenotypes. Here we show that these phenotypes are incurred by a high level of ribonucleotides incorporated by a leading strand-replicase variant, DNA polymerase (Pol) ɛ, but not by orthologous variants of the lagging-strand replicases, Pols α or δ. Moreover, loss of both RNases H1 and H2 is lethal in combination with increased ribonucleotide incorporation by Pol ɛ but not by Pols α or δ. Several explanations for this asymmetry are considered, including the idea that Top1 incision at ribonucleotides relieves torsional stress in the nascent leading strand but not in the nascent lagging strand, in which preexisting nicks prevent the accumulation of superhelical tension.

Place, publisher, year, edition, pages
2015. Vol. 22, no 4, 291-U35 p.
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Basic Medicine
URN: urn:nbn:se:umu:diva-100852DOI: 10.1038/nsmb.2989ISI: 000352317000006PubMedID: 25751426OAI: diva2:794380
Swedish Cancer Society
Available from: 2015-03-11 Created: 2015-03-11 Last updated: 2015-05-21Bibliographically approved

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Marjavaara, LisetteChabes, Andrei
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