Exploring the chicken embryo as a possible model for studying Listeria monocytogenes pathogenicity
2014 (English)In: Frontiers in Cellular and Infection Microbiology, E-ISSN 2235-2988, Vol. 4, 170Article in journal (Refereed) Published
Listeria monocytogenes is a bacterial pathogen capable of causing severe infections in humans, often with fatal outcomes. Many different animal models exist to study L. monocytogenes pathogenicity, and we have investigated the chicken embryo as an infection model: What are the benefits and possible drawbacks? We have compared a defined wild-type strain with its isogenic strains lacking well-characterized virulence factors. Our results show that wild-type L. monocytogenes, already at a relatively low infection dose (similar to 5 x 10(2) cfu), caused death of the chicken embryo within 36 h, in contrast to strains lacking the main transcriptional activator of virulence, PrfA, or the cytolysin LLO. Surprisingly, strains lacking the major adhesins InIA and InIB caused similar mortality as the wild-type strain. In conclusion, our results suggest that the chicken embryo is a practical model to study L. monocytogenes infections, especially when analyzing alternative virulence pathways independent of the InIA and InIB adhesins. However, the route of infection might be different from a human infection. The chicken embryo model and other Listeria infection models are discussed.
Place, publisher, year, edition, pages
2014. Vol. 4, 170
Listeria monocytogenes, chicken embryo, PrfA, virulence: InIA, LLO, InIB
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:umu:diva-100984DOI: 10.3389/fcimb.2014.00170ISI: 000349152600003PubMedID: 25540772OAI: oai:DiVA.org:umu-100984DiVA: diva2:795430