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Myosin light chains and calcium regulating protein differences in chronic musculoskeletal neck and shoulder pain
Umeå University, Faculty of Medicine, Department of Community Medicine and Rehabilitation, Idrottsmedicin.ORCID iD: 0000-0003-4864-7842
Linköpings universitet.
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2015 (English)In: BioMed Research International, ISSN 2314-6133, E-ISSN 2314-6141Article in journal (Refereed) Submitted
Abstract [en]

Understanding the underlying molecular mechanisms behind chronic pain conditions are of importance for treatment and rehabilitation. Global screening analysis has detected myosin light chain (MLC) as a protein implied to be involved in chronic musculoskeletal neck and shoulder pain. Several analyses of MLC proteins have stated a difference in phosphorylation being the determining factor for i.e. senescence. In continuation of a previous publication, this study is an attempt to analyze the different MLC isoforms by mass spectrometry and immune-analyses in myalgic and healthy trapezius muscle. No differences in phosphorylation level between the corresponding individual proteins were detected using LC-MSMS and immunoblotting; instead we assigned different isoforms of regulatory MLCs. To further elucidate the calcium (Ca2+) regulation in muscle two proteins, sarco(endo)plasmic reticulum Ca2+ ATPase 1 (SERCA-1) and calsequestrine (CSQ) were analyzed by western blot. The analysis revealed a significantly increased abundance of SERCA-1 protein in the myalgic muscle and a significantly increased abundance of CSQ in healthy muscle. Myalgic muscle contraction patterns have in previous studies shown to differ from healthy muscle which may be connected to the Ca2+ availability in the muscle. Here we present the proteomic characterization of differences in Ca2+ regulating proteins and particularly regulatory MLCs in trapezius muscle of women with chronic musculoskeletal neck and shoulder pain.

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Keyword [en]
calcium (Ca2+), muscle pain, myosin light chain, trapezius, myalgia
National Category
Environmental Health and Occupational Health
Research subject
URN: urn:nbn:se:umu:diva-101152OAI: diva2:797268
Forte, Swedish Research Council for Health, Working Life and Welfare, 2013-1259
Available from: 2015-03-23 Created: 2015-03-23 Last updated: 2015-03-23

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