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Determination of deoxyribonucleoside triphosphate concentrations in yeast cells by strong anion-exchange high-performance liquid chromatography coupled with ultraviolet detection
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics. (Andrei Chabes)
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics. (Andrei Chabes)
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics. (Andrei Chabes)
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics. (Andrei Chabes)
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2015 (English)In: DNA Replikation: Methods and Protocols / [ed] Sonya Vengrova; Jacob Dalgaard, Warwick Medical School, University of Warwick, New York: Springer-Verlag New York, 2015, Vol. 1300, 113-121 p.Chapter in book (Refereed)
Abstract [en]

DNA polymerase assays are commonly used for the detection of deoxyribonucleoside triphosphates (dNTPs) in biological samples. For better specificity and accuracy, high-performance liquid chromatography (HPLC) methods have been developed for the analysis of the four dNTPs in complex samples. Here we describe a simple method using isocratic strong anion-exchange (SAX) chromatographic separation coupled with ultraviolet detection (UV) for the analysis of the four dNTPs in budding yeast Saccharomyces cerevisiae. This method can be applied to other species of yeast or bacteria.

Place, publisher, year, edition, pages
New York: Springer-Verlag New York, 2015. Vol. 1300, 113-121 p.
Series
Methods in Molecular Biology, ISSN 1064-3745 ; 1300
Keyword [en]
Boronate column separation, Deoxyribonucleoside triphosphates, Liquid chromatography, Strong anion exchange, Budding yeast
National Category
Basic Medicine Medical Genetics
Identifiers
URN: urn:nbn:se:umu:diva-102707DOI: 10.1007/978-1-4939-2596-4_8PubMedID: 25916709ISBN: 978-1-4939-2595-7 (print)ISBN: 978-1-4939-2596-4 (print)OAI: oai:DiVA.org:umu-102707DiVA: diva2:809176
Available from: 2015-04-30 Created: 2015-04-30 Last updated: 2015-10-13Bibliographically approved

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Jia, ShaodongMarjavaara, LisetteBuckland, RobertSharma, SushmaChabes, Andrei
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Department of Medical Biochemistry and BiophysicsMolecular Infection Medicine Sweden (MIMS)
Basic MedicineMedical Genetics

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