Determination of deoxyribonucleoside triphosphate concentrations in yeast cells by strong anion-exchange high-performance liquid chromatography coupled with ultraviolet detection
2015 (English)In: DNA Replikation: Methods and Protocols / [ed] Sonya Vengrova; Jacob Dalgaard, Warwick Medical School, University of Warwick, New York: Springer-Verlag New York, 2015, Vol. 1300, 113-121 p.Chapter in book (Refereed)
DNA polymerase assays are commonly used for the detection of deoxyribonucleoside triphosphates (dNTPs) in biological samples. For better specificity and accuracy, high-performance liquid chromatography (HPLC) methods have been developed for the analysis of the four dNTPs in complex samples. Here we describe a simple method using isocratic strong anion-exchange (SAX) chromatographic separation coupled with ultraviolet detection (UV) for the analysis of the four dNTPs in budding yeast Saccharomyces cerevisiae. This method can be applied to other species of yeast or bacteria.
Place, publisher, year, edition, pages
New York: Springer-Verlag New York, 2015. Vol. 1300, 113-121 p.
, Methods in Molecular Biology, ISSN 1064-3745 ; 1300
Boronate column separation, Deoxyribonucleoside triphosphates, Liquid chromatography, Strong anion exchange, Budding yeast
Basic Medicine Medical Genetics
IdentifiersURN: urn:nbn:se:umu:diva-102707DOI: 10.1007/978-1-4939-2596-4_8PubMedID: 25916709ISBN: 978-1-4939-2595-7 (print)ISBN: 978-1-4939-2596-4 (online)OAI: oai:DiVA.org:umu-102707DiVA: diva2:809176