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Difficulties detecting miRNA-203 in human whole saliva by the use of PCR
Umeå University, Faculty of Medicine, Department of Odontology. Umeå University, Faculty of Medicine, Department of Medical Biosciences.
Umeå University, Faculty of Medicine, Department of Medical Biosciences.
Umeå University, Faculty of Medicine, Department of Odontology. Umeå University, Faculty of Medicine, Department of Medical Biosciences.
2015 (English)In: Medicina Oral, ISSN 1698-4447, E-ISSN 1698-6946, Vol. 20, no 2, E130-E134 p., 20172Article in journal (Refereed) Published
Abstract [en]

Objectives: Oral Lichen Planus (OLP) is a chronic disease of the oral mucosa, and according to the WHO also a pre malignant condition. Micro-RNAs are short non coding RNAs capable of regulating mRNA expression. MiRNA:scan be detected in tissue, blood and human whole saliva (HWS) and recently we have shown miR-203 to be up-regulated in tissue from OLP lesions. Study Design: In order to see whether mRNA as well as miR-203 could be detected also in HWS, saliva from healthy controls and patients with OLP were analysed using two different PCR methods. Results: Results showed low mRNA and miRNA levels in general in HWS samples, making it hard to generate conclusive results. Conclusions: In order to make HWS a valuable source for different analyses, more sensitive PCR techniques capable of detecting very low levels of mRNA and miRNA as well as more efficient methods for extraction of RNA are needed.

Place, publisher, year, edition, pages
2015. Vol. 20, no 2, E130-E134 p., 20172
Keyword [en]
miRNA-203, saliva, PCR
National Category
Dentistry
Identifiers
URN: urn:nbn:se:umu:diva-102380DOI: 10.4317/medoral.20172ISI: 000351923500002PubMedID: 25475777OAI: oai:DiVA.org:umu-102380DiVA: diva2:810309
Available from: 2015-05-07 Created: 2015-04-23 Last updated: 2017-12-04Bibliographically approved

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Lundegard, MartinNylander, KarinDanielsson, Karin

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