Gene expression and fiber type variations in repeated vastus lateralis biopsies
2015 (English)In: Muscle and Nerve, ISSN 0148-639X, E-ISSN 1097-4598, Vol. 52, no 2, 812-817 p.Article in journal (Refereed) Published
Introduction: Muscle sample collection can introduce variation in any measured variable due to inter- and intramuscle variation. We investigated the variation in gene expression and fiber type composition after repeated biopsy sampling from the vastus lateralis muscle. Methods: Six subjects donated 3 tissue samples each. One hour after baseline sampling from 1 vastus lateralis muscle, samples from both vastus lateralis muscles were obtained. Results: The fiber type composition differed between biopsies taken from the same leg. There were no within-subject differences in gene expression between the 3 biopsies. Multivariate analysis supports a model in which gene expression differs significantly between individuals but is not affected by repeated muscle biopsy sampling from the same subject. Conclusion: One vastus lateralis muscle sample per subject is sufficient to establish a reliable baseline for comparing gene expression representing selected pathways over time within the same individual.
Place, publisher, year, edition, pages
2015. Vol. 52, no 2, 812-817 p.
fiber type, gene expression, human, muscle, repeated sampling
IdentifiersURN: urn:nbn:se:umu:diva-103248DOI: 10.1002/mus.24616ISI: 000362549500017PubMedID: 25703336OAI: oai:DiVA.org:umu-103248DiVA: diva2:812485