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The tissue distribution of lipoprotein lipase determines where chylomicrons bind
Umeå University, Faculty of Medicine, Department of Medical Biosciences, Physiological chemistry.
Umeå University, Faculty of Medicine, Department of Medical Biosciences, Physiological chemistry.
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2015 (English)In: Journal of Lipid Research, ISSN 0022-2275, E-ISSN 1539-7262, Vol. 56, no 3, 588-598 p.Article in journal (Refereed) Published
Abstract [en]

To determine the role of LPL for binding of lipoproteins to the vascular endothelium, and for the distribution of lipids from lipoproteins, four lines of induced mutant mice were used. Rat chylomicrons labeled in vivo with [C-14] oleic acid (primarily in TGs, providing a tracer for lipolysis) and [H-3]retinol (primarily in ester form, providing a tracer for the core lipids) were injected. TG label was cleared more rapidly than core label. There were no differences between the mouse lines in the rate at which core label was cleared. Two minutes after injection, about 5% of the core label, and hence chylomicron particles, were in the heart of WT mice. In mice that expressed LPL only in skeletal muscle, and had much reduced levels of LPL in the heart, binding of chylomicrons was reduced to 1%, whereas in mice that expressed LPL only in the heart, the binding was increased to over 10%. The same patterns of distribution were evident at 20 min when most of the label had been cleared. Thus, the amount of LPL expressed in muscle and heart governed both the binding of chylomicron particles and the assimilation of chylomicron lipids in the tissue.

Place, publisher, year, edition, pages
2015. Vol. 56, no 3, 588-598 p.
Keyword [en]
heart, muscle, adipose tissue, liver, triglycerides, nonesterified fatty acids, transgene
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-102469DOI: 10.1194/jlr.M056028ISI: 000350345900011PubMedID: 25589507OAI: oai:DiVA.org:umu-102469DiVA: diva2:812632
Available from: 2015-05-19 Created: 2015-04-26 Last updated: 2017-12-04Bibliographically approved

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Savonen, RogerHultin, MagnusLevak-Frank, SanjaOlivecrona, GunillaOlivecrona, Thomas
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