Regulation of Intracellular Copper by Induction of Endogenous Metallothioneins Improves the Disease Course in a Mouse Model of Amyotrophic Lateral Sclerosis
2015 (English)In: Neurotherapeutics, ISSN 1933-7213, Vol. 12, no 2, 461-476 p.Article in journal (Refereed) Published
Mutations in SOD1 cause amyotrophic lateral sclerosis (ALS), an incurable motor neuron disease. The pathogenesis of the disease is poorly understood, but intracellular copper dyshomeostasis has been implicated as a key process in the disease. We recently observed that metallothioneins (MTs) are an excellent target for the modification of copper dyshomeostasis in a mouse model of ALS (SOD1(G93A)). Here, we offer a therapeutic strategy designed to increase the level of endogenous MTs. The upregulation of endogenous MTs by dexamethasone, a synthetic glucocorticoid, significantly improved the disease course and rescued motor neurons in SOD1(G93A) mice, even if the induction was initiated when peak body weight had decreased by 10 %. Neuroprotection was associated with the normalization of copper dyshomeostasis, as well as with decreased levels of SOD1(G93A) aggregates. Importantly, these benefits were clearly mediated in a MT-dependent manner, as dexamethasone did not provide any protection when endogenous MTs were abolished from SOD1(G93A) mice. In conclusion, the upregulation of endogenous MTs represents a promising strategy for the treatment of ALS linked to mutant SOD1.
Place, publisher, year, edition, pages
2015. Vol. 12, no 2, 461-476 p.
Amyotrophic lateral sclerosis, Copper dyshomeostasis, Dexamethasone, Metallothioneins, Superoxide dismutase-1
IdentifiersURN: urn:nbn:se:umu:diva-103742DOI: 10.1007/s13311-015-0346-xISI: 000353223000018PubMedID: 25761970OAI: oai:DiVA.org:umu-103742DiVA: diva2:815644