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The effect of collagen degradation on chondrocyte volume and morphology in bovine articular cartilage following a hypotonic challenge
Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.
Department of Biomedicine, University of Eastern Finland, Kuopio, Finland; Biocenter Kuopio, University of Eastern Finland, Kuopio, Finland. (Chondrogenic and Osteogenic Differentiation Group)ORCID iD: 0000-0002-6181-9904
Department of Diagnostic Radiology, University of Oulu, Oulu, Finland; Oulu University Hospital, Oulu, Finland.
Human Performance Laboratory, Faculty of Kinesiology, University of Calgary, Calgary, Canada; Mechanical and Manufacturing Engineering, Schulich School of Engineering, University of Calgary, Calgary, Canada; Fischell Department of Bioengineering, Clark School of Engineering, University of Maryland, Maryland, USA.
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2013 (English)In: Biomechanics and Modeling in Mechanobiology, ISSN 1617-7959, E-ISSN 1617-7940, Vol. 12, no 3, 417-429 p.Article in journal (Refereed) Published
Abstract [en]

Collagen degradation is one of the early signs of osteoarthritis. It is not known how collagen degradation affects chondrocyte volume and morphology. Thus, the aim of this study was to investigate the effect of enzymatically induced collagen degradation on cell volume and shape changes in articular cartilage after a hypotonic challenge. Confocal laser scanning microscopy was used for imaging superficial zone chondrocytes in intact and degraded cartilage exposed to a hypotonic challenge. Fourier transform infrared microspectroscopy, polarized light microscopy, and mechanical testing were used to quantify differences in proteoglycan and collagen content, collagen orientation, and biomechanical properties, respectively, between the intact and degraded cartilage. Collagen content decreased and collagen orientation angle increased significantly (p < 0.05) in the superficial zone cartilage after collagenase treatment, and the instantaneous modulus of the samples was reduced significantly (p < 0.05). Normalized cell volume and height 20 min after the osmotic challenge (with respect to the original volume and height) were significantly (p < 0.001 and p < 0.01, respectively) larger in the intact compared to the degraded cartilage. These findings suggest that the mechanical environment of chondrocytes, specifically collagen content and orientation, affects cell volume and shape changes in the superficial zone articular cartilage when exposed to osmotic loading. This emphasizes the role of collagen in modulating cartilage mechanobiology in diseased tissue.

Place, publisher, year, edition, pages
Springer, 2013. Vol. 12, no 3, 417-429 p.
Keyword [en]
articular cartilage, mechanobiology. microscopy, collagen, chondrocyte, enzymatic treatment, osmotic challenge
National Category
Cell and Molecular Biology Biophysics
Research subject
biomechanics; cellforskning
URN: urn:nbn:se:umu:diva-103881DOI: 10.1007/s10237-012-0409-4ISI: 000319289200002PubMedID: 22710890OAI: diva2:816146
Available from: 2015-06-02 Created: 2015-06-02 Last updated: 2015-06-04Bibliographically approved

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Lammi, Mikko
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