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TiO2 coating promotes human mesenchymal stem cell proliferation without the loss of their capacity for chondrogenic differentiation
Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.
Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland.
Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.
Department of Orthopaedics and Traumatology, Kuopio University Hospital, Kuopio, Finland.
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2013 (English)In: Biofabrication, ISSN 1758-5090, Vol. 5, no 2, 025009- p., 23592549Article in journal (Refereed) Published
Abstract [en]

Human mesenchymal stem cells (hMSCs) are used in applications, which may require a large amount of cells; therefore, efficient expansion of the cells is desired. We studied whether TiO2 coating on plastic cell culture dishes could promote proliferation of hMSCs without adverse effects in chondrogenic differentiation. TiO2-films were deposited on polystyrene dishes and glass coverslips using an ultrashort pulsed laser deposition technique. Human MSCs from three donors were expanded on them until 95% confluence, and the cells were evaluated by morphology, immunocytochemistry and quantitative RT-PCR (qRT-PCR). The chondrogenic differentiation in pellets was performed after cultivation on TiO2-coated dishes. Chondrogenesis was evaluated by histological staining of proteoglycans and type II collagen, and qRT-PCR. Human MSC-associated markers STRO-1, CD44, CD90 and CD146 did not change after expansion on TiO2-coated coverslips. However, the cell number after a 48h-culture period was significantly higher on TiO2-coated culture dishes. Importantly, TiO2 coating caused no significant differences in the proteoglycan and type II collagen staining of the pellets, or the expression of chondrocyte-specific genes in the chondrogenesis assay. Thus, the proliferation of hMSCs could be significantly increased when cultured on TiO2-coated dishes without weakening their chondrogenic differentiation capacity. The transparency of TiO2-films allows easy monitoring of the cell growth and morphology under a phase-contrast microscope.

Place, publisher, year, edition, pages
Institute of Physics (IOP), 2013. Vol. 5, no 2, 025009- p., 23592549
Keyword [en]
stem cell biology, biomaterials, thin films, laser deposition, tissue engineeering, growth and division, biological physics
National Category
Cell and Molecular Biology Other Materials Engineering Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
biological chemistry; cellforskning; Materials Science
Identifiers
URN: urn:nbn:se:umu:diva-103883DOI: 10.1088/1758-5082/5/2/025009ISI: 000318149900010PubMedID: 23592549OAI: oai:DiVA.org:umu-103883DiVA: diva2:816152
Available from: 2015-06-02 Created: 2015-06-02 Last updated: 2015-11-18

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Lammi, MikkoQu, Chengjuan
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