Validation and practical evaluation of the Onesenz microdialysis lactate analyser
Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
This study investigated the reliability of the new Onesenz on-line microdialysis lactate analyser compared to the YSI 2300 Stat Plus. Venous blood lactate concentrations were measured simultaneously with both analysers during an incremental cycle ergometer test. Eight physically active men and women completed the test to voluntary exhaustion. A total of 81 blood samples from both devices were included in the analysis. Statistical analysis was conducted through correlation, regression analysis and limits of agreement. Lactate values during graded exercise ranged from 0,5 mM/l to 14,1 mM/l. A significant correlation was observed between the two analysers, r(79)=0,98, p<0,001. Regression analysis determined the equation: [Onesenz] = 0,811*[YSI] + 0,345 and coefficient of determination (R2) was 0,95. The mean difference between the analysers was 0,15 mM/l ± 0,7 mM/l. The agreement between the two analysers deteriorated above 6 mM/l. When studying bLa values above 6 mM/l the mean difference was 1,38 ± 1,3 mM/l. In conclusion, the Onesenz lactate analyser provides reliable bLa measurements up to 6 mM/l compared to the YSI 2300. In the context of sports medicine the more time consuming method of microdialysis limits its appropriateness to longer test protocols, but on the other hand reduces human error. Still, the prototype lacks several features concerning practical usefulness.
Place, publisher, year, edition, pages
Blood lactate, Microdialysis, Lactate threshold, Exercise testing, Lactate analyser
Blodlaktat, mikrodialys, laktattröskel, laktattest, laktatmätare
Sport and Fitness Sciences
IdentifiersURN: urn:nbn:se:umu:diva-104102OAI: oai:DiVA.org:umu-104102DiVA: diva2:817645
Physical Trainer Pogramme
Isaksson, AndreasGilenstam, Kajsa, Med Dr.
Theos, Apostolos, PhD