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Repair of osteochondral defects with recombinant human type II collagen gel and autologous chondrocytes in rabbit
Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland.
Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland.
A. I. Virtanen Institute, University of Eastern Finland, Kuopio, Finland.
Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.
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2013 (English)In: Osteoarthritis and Cartilage, ISSN 1063-4584, E-ISSN 1522-9653, Vol. 21, no 3, 481-490 p., 23257243Article in journal (Refereed) Published
Abstract [en]

Summary

Objective

Recombinant human type II collagen (rhCII) gels combined with autologous chondrocytes were tested as a scaffold for cartilage repair in rabbits in vivo.

Method

Autologous chondrocytes were harvested, expanded and combined with rhCII-gel and further pre-cultivated for 2 weeks prior to transplantation into a 4 mm diameter lesion created into the rabbit's femoral trochlea (n = 8). Rabbits with similar untreated lesions (n = 7) served as a control group.

Results

Six months after the transplantation the repair tissue in both groups filled the lesion site, but in the rhCII-repair the filling was more complete. Both repair groups also had high proteoglycan and type II collagen contents, except in the fibrous superficial layer. However, the integration to the adjacent cartilage was incomplete. The O'Driscoll grading showed no significant differences between the rhCII-repair and spontaneous repair, both representing lower quality than intact cartilage. In the repair tissues the collagen fibers were abnormally organized and oriented. No dramatic changes were detected in the subchondral bone structure. The repair cartilage was mechanically softer than the intact tissue. Spontaneously repaired tissue showed lower values of equilibrium and dynamic modulus than the rhCII-repair. However, the differences in the mechanical properties between all three groups were insignificant.

Conclusion

When rhCII was used to repair cartilage defects, the repair quality was histologically incomplete, but still the rhCII-repairs showed moderate mechanical characteristics and a slight improvement over those in spontaneous repair. Therefore, further studies using rhCII for cartilage repair with emphasis on improving integration and surface protection are required.

Place, publisher, year, edition, pages
Elsevier, 2013. Vol. 21, no 3, 481-490 p., 23257243
Keyword [en]
Cartilage tissue engineering, Collagen, Hydrogel, Recombnant protein, Autologous chondrocyte implantation, Animal model
National Category
Medical and Health Sciences Orthopedics Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
biomechanics; Materials Science; Orthopaedics
Identifiers
URN: urn:nbn:se:umu:diva-104352DOI: 10.1016/j.joca.2012.12.004OAI: oai:DiVA.org:umu-104352DiVA: diva2:819230
Available from: 2015-06-10 Created: 2015-06-10 Last updated: 2017-12-04

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Lammi, MikkoKiviranta, Ilkka

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Lammi, MikkoKiviranta, Ilkka
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Osteoarthritis and Cartilage
Medical and Health SciencesOrthopedicsMedical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

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