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Quantitative analysis of collagen network structure and fibril dimensions in cartilage repair with autologous chondrocyte transplantation.
Department of Anatomy, Institute of Biomedicine, University of Kuopio, Kuopio, Finland; Department of Radiology, Vaasa Central Hospital, Vaasa, Finland.
Department of Orthopaedics and Traumatology, Helsinki University Hospital, Helsinki, Finland.
Department of Anatomy, Institute of Biomedicine, University of Kuopio, Kuopio, Finland.
Department of Biosciences, Applied Biotechnology, University of Kuopio, Kuopio, Finland. (Chondrogenic and Osteogenic Differentiation Group)ORCID iD: 0000-0002-6181-9904
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2010 (English)In: Cells Tissues Organs, ISSN 1422-6405, E-ISSN 1422-6421, Vol. 192, no 6, 351-360 p., 20664251Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE: The aim of this study was to undertake a stereological analysis to quantify the dimensions of the collagen network in the repair tissue of porcine joints after they had been subjected to autologous chondrocyte transplantation (ACT).

METHOD: ACT was used to repair cartilage lesions in knee joints of pigs. Electron-microscopic stereology, immunostaining for type II collagen, and quantitative polarized-light microscopy were utilized to study the collagen fibrils in the repair tissue 3 and 12 months after the operation.

RESULTS: The collagen volume density (V(V)) was lower in the repair tissue than in normal cartilage at 3 months (20.4 vs. 23.7%) after the operation. The collagen surface density (S(V), 1.5·10(-2) vs. 3.1·10(-2) nm(2)/nm(3)) and V(V) increased with time in the repair tissue (20.4 vs. 44.7%). Quantitative polarized-light microscopy detected a higher degree of collagen parallelism in the repair tissue at 3 months after the operation (55.7 vs. 49.7%). In contrast, 1 year after the operation, fibril parallelism was lower in the repair tissue than in the control cartilage (47.5 vs. 69.8%).

CONCLUSION: Following ACT, V(V) and S(V) increased in the repair tissue with time, reflecting maturation of the tissue. One year after the operation, there was a lower level of fibril organization in the repair tissue than in the control cartilage. Thus, the newly synthesized collagen fibrils in the repair tissue appeared to form a denser network than in the control cartilage, but the fibrils remained more randomly oriented.

Place, publisher, year, edition, pages
2010. Vol. 192, no 6, 351-360 p., 20664251
Keyword [en]
articular cartilage, autologous chondrocyte transplantation, electron microscopy, collage, imag analysis
National Category
Cell and Molecular Biology Orthopedics Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Orthopaedics; cellforskning
Identifiers
URN: urn:nbn:se:umu:diva-105400DOI: 10.1159/000319469PubMedID: 20664251OAI: oai:DiVA.org:umu-105400DiVA: diva2:825180
Available from: 2015-06-23 Created: 2015-06-23 Last updated: 2017-12-04

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Lammi, MikkoKiviranta, Ilkka
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Cell and Molecular BiologyOrthopedicsMedical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

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