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Ozone affects growth and development of Pieris brassicae on the wild host plant Brassica nigra
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
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2015 (English)In: Environmental Pollution, ISSN 0269-7491, E-ISSN 1873-6424, Vol. 199, 119-129 p.Article in journal (Refereed) Published
Abstract [en]

When plants are exposed to ozone they exhibit changes in both primary and secondary metabolism, which may affect their interactions with herbivorous insects. Here we investigated the performance and preferences of the specialist herbivore Pieris brassicae on the wild plant Brassica nigra under elevated ozone conditions. The direct and indirect effects of ozone on the plant-herbivore system were studied. In both cases ozone exposure had a negative effect on P. brassicae development. However, in dual-choice tests larvae preferentially consumed plant material previously fumigated with the highest concentration tested, showing a lack of correlation between larval preference and performance on ozone exposed plants. Metabolomic analysis of leaf material subjected to combinations of ozone and herbivore-feeding, and focussing on known defence metabolites, indicated that P. brassicae behaviour and performance were associated with ozone-induced alterations to glucosinolate and phenolic pools. 

Place, publisher, year, edition, pages
2015. Vol. 199, 119-129 p.
Keyword [en]
Brassica nigra, Pieris brassicae, Ozone exposure, Growth and performance, Defence compounds, enolics, Glucosinolates
National Category
Environmental Sciences
Identifiers
URN: urn:nbn:se:umu:diva-102346DOI: 10.1016/j.envpol.2015.01.019ISI: 000351972900016PubMedID: 25645061OAI: oai:DiVA.org:umu-102346DiVA: diva2:825364
Available from: 2015-06-23 Created: 2015-04-23 Last updated: 2017-12-04Bibliographically approved
In thesis
1. Black mustard and the butterfly effect: metabolomics of plant-insect interactions under multiple stress conditions
Open this publication in new window or tab >>Black mustard and the butterfly effect: metabolomics of plant-insect interactions under multiple stress conditions
2017 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

One main goal of ecological research is to understand nature´s complexity, in order to predict the potential impact of environmental perturbations. In this thesis, I investigate the ecological interactions between some of the most ancient organisms living on our planet: plants and insects.

Focus of my research is the interaction between the wild brassicaceous plant black mustard (Brassica nigra L.) and its specialist insect herbivore, the large white cabbage butterfly (Pieris brassicae L). Both organisms are well characterized model species used in chemical ecology research.

Using different analytical techniques, such as liquid and gas chromatography coupled to mass-spectrometry (LC- and GC-MS) and headspace collection of volatile organic compounds (VOCs), I apply the approach of metabolomics and systems biology to the field of ecology to explore the metabolic changes occurring inside the plants exposed to biotic and abiotic stresses.

Particularly, I study the plant metabolic responses against P. brassicae chewing caterpillars during sequential treatment exposure to: abiotic stress by the oxidative air pollutant ozone (O3); dual herbivory with specialist Brevicoryne brassicae piercing-sucking aphids; and chemical induction of plant defences with the oxylipin phytohormone methyl-jasmonate (MeJA).

Results show how during herbivore-induced responses, changes in defence- and growth-metabolic processes are tightly connected to stress protection mechanisms, indicating that plants actively reprogram their inner metabolic networks in order to adapt to consecutive changes in the environment.

This thesis illustrates how evaluating the plant metabolome in its entirety rather than single metabolites, can help us understanding plant responses towards abiotic and biotic stresses, and improve our ability to predict how constant shifts in the environment affect plant physiology and ecology.

 

Abstract [sv]

Ett huvudsyfte för ekologisk forskning är att förstå naturens komplexitet för att kunna förutse effekter av störningar i miljön. I min avhandling har jag fokuserat på ekologiska interaktioner mellan växter och insekter, två av de äldsta terrestra organismgrupperna på jorden.

I mina studier har jag undersökt interaktioner mellan den korsblommiga växten svartsenap (Brassica nigra L.) och den specifika herbivoren kålfjäril (Pieris brassicae L.). Båda är väl karaktäriserade modellarter i kemisk-ekologisk forskning.

De metaboliska förändringar som sker när växten utsätts för biotisk och abiotisk stress har analyserats hjälp av metabolomik, det vill säga analyser av metabolomet i sin helhet med hjälp av tekniker som vätske- och gaskromatografi kopplad till masspektrometri (LC- och GC-MS), och så kallad headspace-uppsamling av flyktiga organiska föreningar (VOCs).

Jag har särskilt undersökt de metaboliska förändringar som sker när växten betas av kålfjärilslarver vid samtidig exponering för: abiotisk stress i form av ozon (O3), en oxidativ luftförorening; ytterligare betning i form av stickande och sugande bladlus (Brevicoryne brassicae); tillsats av oxylipinfytohormon metyl-jasmonat (MeJA), ett ämne som inducerar växtens försvar.

Resultaten visar att de metaboliska förändringar som sker i växten vid herbivori med konsekvenser för dess försvar och tillväxt är nära kopplade till de metaboliska förändringar som sker vid stress, vilket visar att växten kan fortlöpande och aktivt omprogrammera sina metaboliska nätverk för att anpassa sig till förändringar i miljön.

Avhandlingen visar att genom att utvärdera växtmetabolomet i sin helhet, snarare än att studera enskilda metaboliter, vi kan få bättre förståelse för hur växter reagerar på olika former av stress och därmed också bidra till att vi kan göra förutsägelser för hur förändringar i miljön kan påverka växters fysiologi och ekologi.

Place, publisher, year, edition, pages
Umeå: Umeå University, 2017. 70 p.
Keyword
plant physiology, metabolomics, glucosinolates, multiple stress, ozone, Brassica nigra, Pieris brassicae, Brevicoryne brassicae
National Category
Ecology
Research subject
biology; biology, Environmental Science; Molecular Biology; Entomology
Identifiers
urn:nbn:se:umu:diva-134653 (URN)978-91-7601-728-9 (ISBN)
Public defence
2017-06-02, Lilla hörsalen, KB3A9, Kemiskt Biologiskt Centrum (KBC), Umeå, 10:00 (English)
Opponent
Supervisors
Funder
European Science Foundation (ESF), VR/ESF324–2011–787
Available from: 2017-05-12 Created: 2017-05-10 Last updated: 2017-05-12Bibliographically approved

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Papazian, StefanoAlbrectsen, Benedicte R.

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