Expansion of the Chlamydia trachomatis inclusion does not require bacterial replication
2015 (English)In: International Journal of Medical Microbiology, ISSN 1438-4221, E-ISSN 1618-0607, Vol. 305, no 3, 378-382 p.Article in journal (Refereed) Published
Chlamydia trachomatis replication takes place inside of a host cell, exclusively within a vacuole known as the inclusion. During an infection, the inclusion expands to accommodate the increasing numbers of C. trachomatis. However, whether inclusion expansion requires bacterial replication and/or de novo protein synthesis has not been previously investigated in detail. Therefore, using a chemical biology approach, we herein investigated C. trachomatis inclusion expansion under varying conditions in vitro. Under normal cell culture conditions, inclusion expansion correlated with C trachomatis replication. When bacterial replication was inhibited using KSK120: an inhibitor that targets C. trachomatis glucose metabolism, inclusions expanded even in the absence of bacterial replication. In contrast, when bacterial protein synthesis was inhibited using chloramphenicol, expansion of inclusions was blocked. Together, these data suggest that de novo protein synthesis is necessary, whereas bacterial replication is dispensable for C trachomatis inclusion expansion. (C) 2015 The Authors. Published by Elsevier GmbH.
Place, publisher, year, edition, pages
2015. Vol. 305, no 3, 378-382 p.
Chlamydia trachomatis, Chemical biology, Inclusion expansion, Bacterial replication
Microbiology in the medical area
IdentifiersURN: urn:nbn:se:umu:diva-106141DOI: 10.1016/j.ijmm.2015.02.007ISI: 000354583900012PubMedID: 25771502OAI: oai:DiVA.org:umu-106141DiVA: diva2:841306