Improved adherence and spreading of Saos-2 cells on polypropylene surfaces achieved by surface texturing and carbon nitride coating.
2009 (English)In: Journal of materials science. Materials in medicine, ISSN 0957-4530, E-ISSN 1573-4838, Vol. 20, no 11, 2337-2347 p., 19507005Article in journal (Refereed) Published
The adhesion and contact guidance of human primary osteogenic sarcoma cells (Saos-2) were characterized on smooth, microstructured (MST) and micro- and nano-structured (MNST) polypropylene (PP) and on the same samples with a silicon-doped carbon nitride (C(3)N(4)-Si) coating. Injection molding was used to pattern the PP surfaces and the coating was obtained by using ultra-short pulsed laser deposition (USPLD). Surfaces were characterized using atomic force microscopy and surface energy components were calculated according to the Owens-Wendt model. The results showed C(3)N(4)-Si coated surfaces to be significantly more hydrophilic than uncoated ones. In addition, there were 86% more cells in the smooth C(3)N(4)-Si coated PP compared to smooth uncoated PP and 551%/476% more cells with MST/MNST C(3)N(4)-Si coated PP than could be obtained with MST/MNST uncoated PP. Thus the adhesion, spreading and contact guidance of osteoblast-like cells was effectively improved by combining surface texturing and deposition of osteocompatible C(3)N(4)-Si coating.
Place, publisher, year, edition, pages
Springer, 2009. Vol. 20, no 11, 2337-2347 p., 19507005
Surfaces and Interfaces, Thin Films, Characterization and Evaluation of Materials, Metallic Materials, Ceramics, Glass, Composites, Natural Methods, Polymer Sciences, Biomaterials
Medical Materials Biomaterials Science
Research subject cellforskning; Materials Science
IdentifiersURN: urn:nbn:se:umu:diva-106533DOI: 10.1007/s10856-009-3792-3PubMedID: 19507005OAI: oai:DiVA.org:umu-106533DiVA: diva2:842019