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Contrast agent-enhanced computed tomography of articular cartilage: association with tissue composition and properties.
Department of Clinical Neurophysiology, Kuopio University Hospital, Kuopio, Finland; BioMater Center, University of Kuopio, Kuopio, Finland.
Department of Physics, University of Kuopio, Kuopio, Finland.
Department of Physics, University of Kuopio, Kuopio, Finland.
Department of Biosciences, Applied Biotechnology, University of Kuopio, Kuopio, Finland. (Chondrogenic and Osteogenic Differentiation Group)ORCID iD: 0000-0002-6181-9904
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2009 (English)In: Acta Radiologica, ISSN 0284-1851, E-ISSN 1600-0455, Vol. 50, no 1, 78-85 p., 19052932Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Contrast agent-enhanced computed tomography may enable the noninvasive quantification of glycosaminoglycan (GAG) content of articular cartilage. It has been reported that penetration of the negatively charged contrast agent ioxaglate (Hexabrix) increases significantly after enzymatic degradation of GAGs. However, it is not known whether spontaneous degradation of articular cartilage can be quantitatively detected with this technique.

PURPOSE: To investigate the diagnostic potential of contrast agent-enhanced cartilage tomography (CECT) in quantification of GAG concentration in normal and spontaneously degenerated articular cartilage by means of clinical peripheral quantitative computed tomography (pQCT).

MATERIAL AND METHODS: In this in vitro study, normal and spontaneously degenerated adult bovine cartilage (n=32) was used. Bovine patellar cartilage samples were immersed in 21 mM contrast agent (Hexabrix) solution for 24 hours at room temperature. After immersion, the samples were scanned with a clinical pQCT instrument. From pQCT images, the contrast agent concentration in superficial as well as in full-thickness cartilage was calculated. Histological and functional integrity of the samples was quantified with histochemical and mechanical reference measurements extracted from our earlier study.

RESULTS: Full diffusion of contrast agent into the deep cartilage was found to take over 8 hours. As compared to normal cartilage, a significant increase (11%, P<0.05) in contrast agent concentration was seen in the superficial layer of spontaneously degenerated samples. Significant negative correlations were revealed between the contrast agent concentration and the superficial or full-thickness GAG content of tissue (|R| > 0.5, P<0.01). Further, pQCT could be used to measure the thickness of patellar cartilage.

CONCLUSION: The present results suggest that CECT can be used to diagnose proteoglycan depletion in spontaneously degenerated articular cartilage with a clinical pQCT scanner. Possibly, the in vivo use of clinical pQCT for CECT arthrography of human joints is feasible.

Place, publisher, year, edition, pages
Informa Healthcare, 2009. Vol. 50, no 1, 78-85 p., 19052932
Keyword [en]
Articular cartilage, osteoarthritis, x-ray tomography, contrast agent
National Category
Radiology, Nuclear Medicine and Medical Imaging Orthopedics
Research subject
Orthopaedics; Radiology
URN: urn:nbn:se:umu:diva-106540DOI: 10.1080/02841850802572526PubMedID: 19052932OAI: diva2:842196
Available from: 2015-07-17 Created: 2015-07-17 Last updated: 2015-07-17

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