Change search
ReferencesLink to record
Permanent link

Direct link
Nitrogen limitation of heterotrophic biofilms in boreal streams
Umeå University, Faculty of Science and Technology, Department of Ecology and Environmental Sciences.
Umeå University, Faculty of Science and Technology, Department of Ecology and Environmental Sciences. (Arcum)
Show others and affiliations
2015 (English)In: Freshwater Biology, ISSN 0046-5070, E-ISSN 1365-2427, Vol. 60, no 7, 1237-1251 p.Article in journal (Refereed) Published
Abstract [en]

Nutrient limitation of the biofilm is fundamental to stream ecosystem processes, as microbial activity shapes the biological availability and biogeochemical cycling of carbon and nutrients. We used nutrient-diffusing substrata (NDS) to investigate heterotrophic nutrient limitation of microbial respiration (MR) across 20 streams draining boreal landscapes in northern Sweden. We also explored variation in microbial biomass and community structure of biofilms that developed on NDS using phospholipid fatty acid (PLFA) biomarkers. Limitation was determined as a significant response of MR and biomass production on cellulose surfaces to enrichment with nitrogen (N), phosphorus (P) or N+P, relative to controls. Microbial respiration was N-limited, with an average 3.3-fold increase on N-amended NDS. Nitrogen limitation decreased, and control rates of MR increased, with greater background concentrations of inorganic N across the sites. In contrast to MR, microbial biomass was primarily N-limited but was greatest for the N+P NDS. Accordingly, differences in respiratory versus biomass responses to nutrient addition resulted in significantly greater biomass-specific MR on N-amended NDS compared to all other treatments. In addition, PLFA biomarkers indicated distinct microbial communities on N and N+P NDS compared to controls and/or P NDS. Greater MR and biomass, and the development of distinct microbial communities, when supplied with inorganic N suggest that factors which alter aquatic N loading during autumn may have important implications for ecosystem processes and the biogeochemistry of boreal streams and rivers. Our findings add to a growing body of evidence that the productivity of Fennoscandian boreal landscapes is constrained by N availability.

Place, publisher, year, edition, pages
2015. Vol. 60, no 7, 1237-1251 p.
Keyword [en]
biofilms, microbial processing, nutrient limitation, phospholipid fatty acid, respiration
National Category
URN: urn:nbn:se:umu:diva-106316DOI: 10.1111/fwb.12549ISI: 000356370800001OAI: diva2:842272
Available from: 2015-07-17 Created: 2015-07-10 Last updated: 2016-06-21Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Hotchkiss, Erin R.Jonsson, MicaelSponseller, Ryan A.
By organisation
Department of Ecology and Environmental Sciences
In the same journal
Freshwater Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 147 hits
ReferencesLink to record
Permanent link

Direct link