cDNA array reveals mechanosensitive genes in chondrocytic cells under hydrostatic pressure.
2002 (English)In: Biochimica et Biophysica Acta, ISSN 0006-3002, Vol. 1591, no 1-3, 45-54 p., 12183054Article in journal (Refereed) Published
Hydrostatic pressure (HP) has a profound effect on cartilage metabolism in normal and pathological conditions, especially in weight-bearing areas of the skeletal system. As an important component of overall load, HP has been shown to affect the synthetic capacity and well-being of chondrocytes, depending on the mode, duration and magnitude of pressure. In this study we examined the effect of continuous HP on the gene expression profile of a chondrocytic cell line (HCS-2/8) using a cDNA array containing 588 well-characterized human genes under tight transcriptional control. A total of 51 affected genes were identified, many of them not previously associated with mechanical stimuli. Among the significantly up-regulated genes were immediate-early genes, and genes involved in heat-shock response (hsp70, hsp40, hsp27), and in growth arrest (GADD45, GADD153, p21(Cip1/Waf1), tob). Markedly down-regulated genes included members of the Id family genes (dominant negative regulators of basic helix-loop-helix transcription factors), and cytoplasmic dynein light chain and apoptosis-related gene NIP3. These alterations in the expression profile induce a transient heat-shock gene response and activation of genes involved in growth arrest and cellular adaptation and/or differentiation.
Place, publisher, year, edition, pages
Elsevier, 2002. Vol. 1591, no 1-3, 45-54 p., 12183054
Human chondrosarcoma, hydrostatic pressure, mechanobiology, gene expression profiling, microarray
Cell and Molecular Biology Biochemistry and Molecular Biology
Research subject Biochemistry; cellforskning; Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-107058DOI: 10.1016/S0167-4889(02)00247-1PubMedID: 12183054OAI: oai:DiVA.org:umu-107058DiVA: diva2:846804