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cDNA array reveals mechanosensitive genes in chondrocytic cells under hydrostatic pressure.
Department of Anatomy, University of Kuopio, Kuopio, Finland.
Department of Anatomy, University of Kuopio, Kuopio, Finland.
Department of Anatomy, University of Kuopio, Kuopio, Finland.
Department of Anatomy, University of Kuopio, Kuopio, Finland.
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2002 (English)In: Biochimica et Biophysica Acta, ISSN 0006-3002, E-ISSN 1878-2434, Vol. 1591, no 1-3, 45-54 p., 12183054Article in journal (Refereed) Published
Abstract [en]

Hydrostatic pressure (HP) has a profound effect on cartilage metabolism in normal and pathological conditions, especially in weight-bearing areas of the skeletal system. As an important component of overall load, HP has been shown to affect the synthetic capacity and well-being of chondrocytes, depending on the mode, duration and magnitude of pressure. In this study we examined the effect of continuous HP on the gene expression profile of a chondrocytic cell line (HCS-2/8) using a cDNA array containing 588 well-characterized human genes under tight transcriptional control. A total of 51 affected genes were identified, many of them not previously associated with mechanical stimuli. Among the significantly up-regulated genes were immediate-early genes, and genes involved in heat-shock response (hsp70, hsp40, hsp27), and in growth arrest (GADD45, GADD153, p21(Cip1/Waf1), tob). Markedly down-regulated genes included members of the Id family genes (dominant negative regulators of basic helix-loop-helix transcription factors), and cytoplasmic dynein light chain and apoptosis-related gene NIP3. These alterations in the expression profile induce a transient heat-shock gene response and activation of genes involved in growth arrest and cellular adaptation and/or differentiation.

Place, publisher, year, edition, pages
Elsevier, 2002. Vol. 1591, no 1-3, 45-54 p., 12183054
Keyword [en]
Human chondrosarcoma, hydrostatic pressure, mechanobiology, gene expression profiling, microarray
National Category
Cell and Molecular Biology Biochemistry and Molecular Biology
Research subject
Biochemistry; cellforskning; Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-107058DOI: 10.1016/S0167-4889(02)00247-1PubMedID: 12183054OAI: oai:DiVA.org:umu-107058DiVA: diva2:846804
Available from: 2015-08-18 Created: 2015-08-18 Last updated: 2017-12-04

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