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Mutations of ribosomal protein S5 suppress a defect in late-30S ribosomal subunit biogenesis caused by lack of the RbfA biogenesis factor
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Virology. Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Baltimore, Maryland 21228, USA.
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology).
Baltimore, Maryland 21228, USA.
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2015 (English)In: RNA: A publication of the RNA Society, ISSN 1355-8382, E-ISSN 1469-9001, Vol. 21, no 8, 1454-1468 p.Article in journal (Refereed) Published
Abstract [en]

The in vivo assembly of ribosomal subunits requires assistance by maturation proteins that are not part of mature ribosomes. One such protein, RbfA, associates with the 30S ribosomal subunits. Loss of RbfA causes cold sensitivity and defects of the 30S subunit biogenesis and its overexpression partially suppresses the dominant cold sensitivity caused by a C23U mutation in the central pseudoknot of 16S rRNA, a structure essential for ribosome function. We have isolated suppressor mutations that restore partially the growth of an RbfA-lacking strain. Most of the strongest suppressor mutations alter one out of three distinct positions in the carboxy-terminal domain of ribosomal protein S5 (S5) in direct contact with helix 1 and helix 2 of the central pseudoknot. Their effect is to increase the translational capacity of the RbfA-lacking strain as evidenced by an increase in polysomes in the suppressed strains. Overexpression of RimP, a protein factor that along with RbfA regulates formation of the ribosome's central pseudoknot, was lethal to the RbfA-lacking strain but not to a wild-type strain and this lethality was suppressed by the alterations in S5. The S5 mutants alter translational fidelity but these changes do not explain consistently their effect on the RbfA-lacking strain. Our genetic results support a role for the region of S5 modified in the suppressors in the formation of the central pseudoknot in 16S rRNA.

Place, publisher, year, edition, pages
Cold Spring Harbor Laboratory Press (CSHL), 2015. Vol. 21, no 8, 1454-1468 p.
Keyword [en]
ribosome assembly, 16S rRNA central pseudoknot, RbfA, RimP, translational accuracy
National Category
Cell and Molecular Biology
URN: urn:nbn:se:umu:diva-106776DOI: 10.1261/rna.051383.115ISI: 000358016000007PubMedID: 26089326OAI: diva2:847810
Available from: 2015-08-21 Created: 2015-08-07 Last updated: 2015-12-11Bibliographically approved

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Nord, StefanTükenmez, HasanWikström, P Mikael
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VirologyDepartment of Molecular Biology (Faculty of Medicine)Department of Molecular Biology (Faculty of Science and Technology)
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