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Type X collagen in human enamel development: a possible role in mineralization.
Department of Anatomy, Histology and Embryology, University of Debrecen Medical and Health Science Center, Debrecen, Hungary.
Department of Anatomy, Histology and Embryology, University of Debrecen Medical and Health Science Center, Debrecen, Hungary.
Department of Anatomy, Histology and Embryology, University of Debrecen Medical and Health Science Center, Debrecen, Hungary.
Department of Anatomy, University of Kuopio, Kuopio, Finland. (Chondrogenic and Osteogenic Differentiation Group)ORCID iD: 0000-0002-6181-9904
2000 (English)In: Acta Odontologica Scandinavica, ISSN 0001-6357, E-ISSN 1502-3850, Vol. 58, no 4, 171-176 p., 11045371Article in journal (Refereed) Published
Abstract [en]

Although type X collagen is one of the key molecules in endochondral ossification, no data are available on whether it is present in dental structures when mineralization is proceeding. We therefore monitored the appearance of type X collagen in tooth germs of human samples ranging in gestational age from 17-week-old fetuses to 9-week-old newborn. Using immunohistochemistry, ELISA techniques, and Western blotting, we show that type X collagen is present in human tooth germ during enamel maturation. Intense immunohistochemical staining for collagen type X was observed in the enamel and in the apical parts of secretory ameloblast at the bell stage when the dentine and enamel matrix were already under formation. The odontoblasts, the dentine, and the pulp were not stained. In the early (9-week) postnatal stage, the staining for collagen type X in the enamel matrix was diminished, and only a very weak signal could be detected in the secretory ameloblasts. A positive reaction for collagen type X was also observed in ELISA assay of extracts obtained from human embryonic enamel and hypertrophic cartilage samples. The Western blot analysis of the enamel demonstrated that size of the molecule detected by MoAb X53 is characteristic of the type X collagen. This correlates well with our immunohistochemical findings. Based on these data, we propose that type X collagen is one of the candidate molecules present in the enamel matrix that might be involved in mineralization of the enamel.

Place, publisher, year, edition, pages
Informa Healthcare, 2000. Vol. 58, no 4, 171-176 p., 11045371
Keyword [en]
Human, tooth, enamel, collagen type X, mineralization
National Category
Dentistry
Research subject
Odontology; Biochemistry
Identifiers
URN: urn:nbn:se:umu:diva-107613PubMedID: 11045371OAI: oai:DiVA.org:umu-107613DiVA: diva2:848472
Available from: 2015-08-25 Created: 2015-08-25 Last updated: 2017-12-04

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