Type X collagen, a natural component of mouse articular cartilage: association with growth, aging, and osteoarthritis.
1998 (English)In: Arthritis and Rheumatism, ISSN 0004-3591, E-ISSN 1529-0131, Vol. 41, no 7, 1287-1295 p., 9663487Article in journal (Refereed) Published
OBJECTIVE: To perform a systematic study on the production and deposition of type X collagen in developing, aging, and osteoarthritic (OA) mouse articular cartilage.
METHODS: Immunohistochemistry was employed to define the distribution of type X collagen and Northern analyses to determine the messenger RNA levels as an indicator of the synthetic activity of the protein.
RESULTS: Type X collagen was observed in the epiphyseal and articular cartilage of mouse knee joints throughout development and growth. Type X collagen deposition in the transitional zone of articular cartilage became evident toward cessation of growth, at the age of 2-3 months. The most intense staining for type X collagen was limited to the tidemark, the border between uncalcified and calcified cartilage. Northern analysis confirmed that the type X collagen gene is also transcribed by articular cartilage chondrocytes. Intense immunostaining was observed in the areas of OA lesions, specifically, at sites of osteophyte formation and surface fibrillation. Type X collagen deposition was also seen in degenerating menisci.
CONCLUSION: This study demonstrates that type X collagen is a natural component of mouse articular cartilage throughout development, growth, and aging. This finding and the deposition of type X collagen at sites of OA lesions suggest that type X collagen may have a role in providing structural support for articular cartilage.
Place, publisher, year, edition, pages
John Wiley & Sons, 1998. Vol. 41, no 7, 1287-1295 p., 9663487
Articular cartilge, mouse, type X collagen, growth, ageing
Biochemistry and Molecular Biology Cell and Molecular Biology
Research subject Biochemistry; Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-107665DOI: 10.1002/1529-0131(199807)41:7<1287::AID-ART20>3.0.CO;2-DPubMedID: 9663487OAI: oai:DiVA.org:umu-107665DiVA: diva2:848742