Change search
ReferencesLink to record
Permanent link

Direct link
Coordinated regulation of hyaluronan and aggrecan content in the articular cartilage of immobilized and exercised dogs.
Department of Surgery, Kuopio University Hospital, Kuopio, Finland.
Department of Anatomy, University of Kuopio, Kuopio, Finland. (Chondrogenic and Osteogenic Differentiation Group)ORCID iD: 0000-0002-6181-9904
Department of Anatomy, University of Kuopio, Kuopio, Finland.
Department of Anatomy, University of Kuopio, Kuopio, Finland.
Show others and affiliations
1996 (English)In: Journal of Rheumatology, ISSN 0315-162X, E-ISSN 1499-2752, Vol. 23, no 9, 1586-1593 p., 8877929Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE: To study the influence of joint loading and immobilization on articular cartilage hyaluronan concentration and histological distribution in the knee joints of young dogs subjected to 11 weeks' immobilization by splinting, and 15 weeks' running exercise at a rate of 40 km/day.

METHODS: The amount of hyaluronan in articular cartilage was determined by a competitive binding assay using a biotinylated hyaluronan binding complex (HABC) of aggrecan and link protein. Histologic sections were stained for the localization of hyaluronan with the HABC probe. Extracted proteoglycans were characterized by sodium dodecyl sulfate agarose gel electrophoresis.

RESULTS: Immobilization significantly reduced the concentration of hyaluronan in all sites studied (tibial and femoral condyles, patellar surface of femur). The proportion of hyaluronan to total uronic acid (mainly from aggrecan) remained unchanged because of a concurrent decrease in aggrecan. The ratio of hyaluronan and aggrecan remained constant also in runners. The staining pattern of free hyaluronan in the tissue sections and the electrophoretic mobility of the extracted proteoglycans were not affected by the different loading regimes.

CONCLUSION: Reduced joint loading due to splint immobilization significantly decreases both hyaluronan and aggrecan in the articular cartilage. The remarkably parallel changes in aggrecan and hyaluronan content suggest that joint loading exerts a coordinated influence on their metabolism.

Place, publisher, year, edition, pages
Journal of Rheumatology , 1996. Vol. 23, no 9, 1586-1593 p., 8877929
Keyword [en]
Articular cartilage, immobilization, exercise, hyaluronan, proteogylcans, dog
National Category
Orthopedics Cell and Molecular Biology Biochemistry and Molecular Biology
Research subject
Biochemistry; cellforskning; Orthopaedics
URN: urn:nbn:se:umu:diva-108051PubMedID: 8877929OAI: diva2:850747
Available from: 2015-09-02 Created: 2015-09-02 Last updated: 2015-09-02

Open Access in DiVA

No full text


Search in DiVA

By author/editor
Lammi, Mikko
In the same journal
Journal of Rheumatology
OrthopedicsCell and Molecular BiologyBiochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 41 hits
ReferencesLink to record
Permanent link

Direct link