Proteoglycans in the intervertebral disc of young dogs following strenuous running exercise.
1994 (English)In: Connective Tissue Research, ISSN 0300-8207, E-ISSN 1607-8438, Vol. 30, no 3, 225-240 p., 8039389Article in journal (Refereed) Published
The proteoglycans (PGs) of intervertebral disc were studied in ten beagles which ran on a treadmill for one year (up to 40 km/day) and in ten non-running control dogs. Nucleus pulposus and annulus fibrosus from cervical (C5) and thoracic (T6 and T12) discs were labeled in vitro with 35SO4. The extractability, concentration and synthesis of PGs, and the electrophoretic subpopulations, aggregation and glycosaminoglycan chain lengths of newly-synthesized and total PGs were measured. Sulfate incorporation was significantly elevated by running in the C5 disc and reduced in the annulus of T6 discs. In the annulus of the T6 discs the concentration of total PGs was significantly lower although that of dermatan sulfate PGs was actually higher than in the controls. The results show that enhanced loading of the spine exerts significant alterations in the intervertebral disc PGs in a spine-level specific manner. In the most strained area of the spine (upper thoracic), the alterations in the runners suggest compromised biomechanical properties of the disc.
Place, publisher, year, edition, pages
Informa Healthcare, 1994. Vol. 30, no 3, 225-240 p., 8039389
Intervertebral disc, exercise, proteoglycan, dog
Orthopedics Biochemistry and Molecular Biology
Research subject Biochemistry; Orthopaedics
IdentifiersURN: urn:nbn:se:umu:diva-108068DOI: 10.3109/03008209409061974PubMedID: 8039389OAI: oai:DiVA.org:umu-108068DiVA: diva2:850894