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Proteoglycan synthesis in canine intervertebral discs after long-distance running training.
Department of Anatomy, University of Kuopio, Kuopio, Finland.
Department of Anatomy, University of Kuopio, Kuopio, Finland. (Chondrogenic and Osteogenic Differentiation Group)ORCID iD: 0000-0002-6181-9904
Department of Surgery, Kuopio University Hospital, Kuopio, Finland.
Department of Anatomy, University of Kuopio, Kuopio, Finland.
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1993 (English)In: Journal of Orthopaedic Research, ISSN 0736-0266, E-ISSN 1554-527X, Vol. 11, no 5, 738-746 p., 8410474Article in journal (Refereed) Published
Abstract [en]

The alterations and distribution of proteoglycan (PG) synthesis in the intervertebral discs of young dogs exercised with long-distance running (40 km/day) were studied with a method based on image analysis of tissue sections. Ten dogs were run on a treadmill daily for 55 weeks, and 10 dogs from the same litters served as controls. The daily running distance gradually was increased to 40 km and was maintained at that level for the final 15 weeks. Midsagittal disc segments C7-T1, T8-9, and L1-2 were labeled with 35SO4, and histological sections of the segments were apposed against autoradiographic film to determine the synthesis of PGs. Next, the same sections were stained with safranin O to estimate possible alterations in PG concentration. The radiographs and stained sections were digitized with a flatbed scanner and measured by image analysis. The lumbar discs of runners displayed a significantly lower rate of 35SO4 incorporation, while a tendency toward enhanced incorporation was seen in the cervical and thoracic discs. Safranin O staining showed a pattern just opposite to 35SO4 incorporation: decreased staining in the cervical and thoracic discs and increased staining in the lumbar discs of the runners. The results demonstrate qualitatively different influences of long-term running training on PG metabolism at the cervical, thoracic, and lumbar levels in young dogs.

Place, publisher, year, edition, pages
John Wiley & Sons, 1993. Vol. 11, no 5, 738-746 p., 8410474
Keyword [en]
Intervertebral disc, proteoglycans, exercise, dog
National Category
Orthopedics Biochemistry and Molecular Biology
Research subject
Biochemistry; Orthopaedics
URN: urn:nbn:se:umu:diva-108494DOI: 10.1002/jor.1100110516PubMedID: 8410474OAI: diva2:853213
Available from: 2015-09-11 Created: 2015-09-11 Last updated: 2015-09-11

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