Differences in root functions during long-term drought adaptation: comparison of active gene sets of two wheat genotypes
2010 (English)In: Plant Biology, ISSN 1435-8603, E-ISSN 1438-8677, Vol. 12, no 6, 871-882 p.Article in journal (Refereed) Published
In an attempt to shed light on the role of root systems in differential responses of wheat genotypes to long-term water limitation, transcriptional differences between two wheat genotypes (Triticum aestivum L., cv. Plainsman V and landrace Kobomugi) were identified during adaptation to moderate water stress at the tillering stage. Differences in organ sizes, water-use efficiency and seed production were detected in plants grown in soil, and root functions were characterised by expression profiling. The molecular genetic background of the behaviour of the two genotypes during this stress was revealed using a cDNA macroarray for transcript profiling of the roots. During a 4-week period of moderate water deficit, a set of up-regulated genes displaying transiently increased expression was identified in young plantlets, mostly in the second week in the roots of Kobomugi, while transcript levels remained constantly high in roots of Plainsman V. These genes encode proteins with various functions, such as transport, protein metabolism, osmoprotectant biosynthesis, cell wall biogenesis and detoxification, and also regulatory proteins. Oxidoreductases, peroxidases and cell wall-related genes were induced significantly only in Plainsman V, while induction of stress- and defence-related genes was more pronounced in Kobomugi. Real-time qPCR analysis of selected members of the glutathione S-transferase gene family revealed differences in regulation of family members in the two genotypes and confirmed the macroarray results. The TaGSTZ gene was stress-activated only in the roots of Kobomugi.
Place, publisher, year, edition, pages
Wiley-Blackwell, 2010. Vol. 12, no 6, 871-882 p.
Drought tolerance, glutathione S-transferase, macroarray, real-time qPCR, tillering stage, Triticum aestivum L
IdentifiersURN: urn:nbn:se:umu:diva-109731DOI: 10.1111/j.1438-8677.2009.00295.xISI: 000282817700005PubMedID: 21040302OAI: oai:DiVA.org:umu-109731DiVA: diva2:859101