umu.sePublications
Change search
ReferencesLink to record
Permanent link

Direct link
Toll-Like Receptor 2 Stimulation on Osteoblasts Mediates Staphylococcus aureus-Induced Bone Resorption and Osteoclastogenesis through Enhanced RANKL
Umeå University, Faculty of Medicine, Department of Odontology.
Umeå University, Faculty of Medicine, Department of Odontology.
(English)Manuscript (preprint) (Other academic)
Abstract [en]

Severe Staphylococcus aureus (S. aureus) infections pose an immense threat to population health and constitute a great burden for the health care worldwide. Inter alia, S. aureus septic arthritis is a disease with high mortality and morbidity caused by destruction of the infected joints and systemic bone loss, osteoporosis. Toll-Like receptors (TLRs) are innate immune cell receptors recognizing a variety of microbial molecules and structures. S. aureus recognition via TLR2 initiates a signaling cascade resulting in production of various cytokines, but the mechanisms by which S. aureus causes rapid and excessive bone loss are still unclear. Therefore, we investigated how S. aureus regulates periosteal/endosteal osteoclast formation and bone resorption.

S. aureus stimulation of neonatal mouse parietal bone induced ex vivo bone resorption and osteoclastic gene expression. This effect was associated with increased mRNA and protein expression of receptor activator of NF-kB ligand (RANKL) without significant change in osteoprotegerin (OPG) expression. S. aureus also increased the expression of proinflammatory cytokines and prostaglandins in parietal bones but the stimulatory effect of S. aureus on bone resorption and Tnfsf11 mRNA expression was independent of these cytokines and prostaglandins. Stimulation of isolated periosteal osteoblasts with S. aureus also resulted in increased expression of Tnfsf11 mRNA, an effect lost in osteoblasts from Tlr2 knockout mice. S. aureus stimulated osteoclastogenesis in isolated periosteal cells without affecting RANKL-stimulated resorption.  In contrast, S. aureus inhibited RANKL-induced osteoclast formation in bone marrow macrophages. These data show that S. aureus enhances bone resorption and periosteal osteoclast formation by increasing osteoblast RANKL production through TLR2. In contrast, S. aureus inhibits osteoclastogenesis from bone marrow derived precursors. Our study indicates the importance of using different in vitro approaches for studies of regulation of osteoclastogenesis by S. aureus to obtain better understanding of the complex mechanisms of S. aureus bone destruction in vivo.

National Category
Dentistry
Identifiers
URN: urn:nbn:se:umu:diva-110287OAI: oai:DiVA.org:umu-110287DiVA: diva2:861975
Available from: 2015-10-19 Created: 2015-10-19 Last updated: 2015-10-20
In thesis
1. Toll-like receptors (TLRs) and inflammatory bone modeling
Open this publication in new window or tab >>Toll-like receptors (TLRs) and inflammatory bone modeling
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Toll-liknande receptorer och inflammatorisk benmodellering
Abstract [en]

Patients with inflammatory or infectious conditions such as periodontitis, peri-implantitis, osteomyelitis, rheumatoid arthritis, septic arthritis and loosened joint prosthesis display varying severity of destruction in the adjacent bone tissue. Bone loss in inflammatory diseases is considered a consequence of cytokine induced RANKL and subsequent enhanced osteoclast formation. Hence, osteotropic cytokines and their receptors have been suggested to be important for the pathogenesis of inflammation-induced osteolysis. It is, here, suggested that bacterial components, so called “pathogen associated molecular patterns=PAMPs”, may also be involved. Varieties of cells express receptors for PAMPs, including Toll-like receptors (TLRs) which are the first line of defence in the innate immune system. LPS (lipopolysaccharide), fimbria and lipoproteins from pathogenic bacteria such as P. gingivalis, S. aureus are ligands for TLR2 and flagellin from pathogenic flagellated bacteria like S. typhimurium is a ligand for TLR5.

 

Since the susceptibility to, or the severity of inflammation-associated bone diseases are likely related to differences in the tissue response, and the mechanisms by which PAMPs interact with bone cells are not fully understood, we aimed to elucidate the importance of different TLRs for inflammation induced bone loss by conducting in vitro and in vivo investigations.

Activation of TLR2 and TLR5 in organ cultured mouse parietal bones increased bone resorption in a time- and concentration-dependent manner by a process inhibited by OPG and bisphosphonate, showing the crucial role of RANKL-induced osteoclast formation. In addition, the number of osteoclasts, expression of osteoclastic genes and osteoclastogenic transcription factors were increased. In the bones and in osteoblasts isolated from the bones, TLR2 agonists increased the expression of RANKL without affecting OPG, while TLR5 activation resulted in enhanced RANKL and decreased OPG. Activation of both TLR2 and TLR5 stimulated the expression in both bones and osteoblasts of prostaglandins and pro-inflammatory cytokines, known to stimulate RANKL. By blocking the cytokines and prostaglandin, we showed that TLR2 and TLR5 induced bone resorption and RANKL expression are independent of these molecules.

Activation of TLR2, but not TLR5, in mouse bone marrow macrophage cultures inhibited RANKL-induced osteoclast formation, an effect not observed in committed pre-osteoclasts.

Local administration in vivo of TLR2 and TLR5 agonists on the top of mouse skull bones enhanced local and systemic osteoclast formation and bone resorption. Using knockout mice, we showed that the effects by LPS from P. gingivalis (used as TLR2 agonist) and flagellins (used as TLR5 agonists) are explicit for TLR2 and TLR5 ex vivo and in vivo, respectively.

These data show that stimulation of TLR2 and TLR5 results in bone resorption in vitro and in vivo mediated by increased RANKL in osteoblasts and thus may be one mechanism for developing inflammatory bone loss.

Interestingly, histological analyses of skull bones of mice treated locally with TLR2 and TLR5 agonists revealed that the bones not only reacted with locally increased osteoclastogenesis (osteoclast formation), but also with locally increased new bone formation. This was observed on both periosteal and endosteal sides of the bones, as well as in the bone marrow compartment. The formation of new bone was seen close to osteoclasts in some parts, but also in other areas, distant from these cells. The response was associated with active, cuboidal osteoblasts, extensive cell proliferation and increased expression of genes coding for bone matrix proteins and osteoblastic transcription factors.

In conclusion, activation of TLR2 and TLR5 in osteoblasts results in bone loss associated with enhanced osteoclast formation and bone resorption, as well as with increased osteoblast differentiation and new bone formation, indicating that inflammation causes bone modeling. The data provide an explanation why LPS from P. gingivalis and flagellin from flagella-expressing bacteria can stimulate bone loss. Since TLR2 and TLR5 can be activated not only by bacterial components, but also by endogenous ligands produced in inflammatory processes, the data also contribute to the understanding of inflammation induced bone loss in autoimmune diseases. Hopefully, these findings will contribute to the development of treatment strategies for inflammation induced bone loss.

Place, publisher, year, edition, pages
Umeå: Umeå University, 2015. 133 p.
Series
Umeå University odontological dissertations, ISSN 0345-7532 ; 134
Keyword
Toll-like receptor, osteoclast, osteoblast, bone resorption, bone formation, P. gingivalis, S. aureus, flagellin.
National Category
Cell and Molecular Biology Dentistry
Identifiers
urn:nbn:se:umu:diva-110296 (URN)978-91-7601-370-0 (ISBN)
Public defence
2015-11-11, Hörsal B, Tandläkarhögskolan, plan 9, Norrlands universitetssjukhus§, Umeå, 09:00 (English)
Opponent
Supervisors
Funder
Swedish Research Council
Available from: 2015-10-21 Created: 2015-10-19 Last updated: 2015-10-22Bibliographically approved

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Kassem, AliLerner, Ulf
By organisation
Department of Odontology
Dentistry

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 539 hits
ReferencesLink to record
Permanent link

Direct link