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The multicopy sRNA LhrC controls expression of the oligopeptide-binding protein OppA in Listeria monocytogenes
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2015 (English)In: RNA Biology, ISSN 1547-6286, E-ISSN 1555-8584, Vol. 12, no 9, 985-997 p.Article in journal (Refereed) Published
Abstract [en]

Listeria monocytogenes is the causative agent of the foodborne disease listeriosis. During infection, L. monocytogenes produces an array of non-coding RNAs, including the multicopy sRNA LhrC. These five, nearly identical sRNAs are highly induced in response to cell envelope stress and target the virulence adhesin lapB at the post-transcriptional level. Here, we demonstrate that LhrC controls expression of additional genes encoding cell envelope-associated proteins with virulence function. Using transcriptomics and proteomics, we identified a set of genes affected by LhrC in response to cell envelope stress. Three targets were significantly down-regulated by LhrC at both the RNA and protein level: lmo2349, tcsA and oppA. All three genes encode membrane-associated proteins: A putative substrate binding protein of an amino acid ABC transporter (Lmo2349); the CD4+ T cell-stimulating antigen TcsA, and the oligopeptide binding protein OppA, of which the latter 2 are required for full virulence of L. monocytogenes. For OppA, we show that LhrC acts by direct base paring to the ribosome binding site of the oppA mRNA, leading to an impediment of its translation and a decreased mRNA level. The sRNA-mRNA interaction depends on 2 of 3 CU-rich regions in LhrC allowing binding of 2 oppA mRNAs to a single LhrC molecule. Finally, we found that LhrC contributes to infection in macrophage-like cells. These findings demonstrate a central role for LhrC in controlling the level of OppA and other virulence-associated cell envelope proteins in response to cell envelope stress.

Place, publisher, year, edition, pages
[Sievers, Susanne; Lund, Anja; Menendez-Gil, Pilar; Nielsen, Aaraby; Storm Mollerup, Maria; Lambert Nielsen, Stine; Buch Larsson, Pernille; Borch-Jensen, Jonas; Kallipolitis, Birgitte Haahr] Univ Southern Denmark, Dept Biochem & Mol Biol, Odense, Denmark. [Sievers, Susanne] Ernst Moritz Arndt Univ Greifswald, Inst Microbiol, Greifswald, Germany. [Johansson, Joergen] Umea Univ, Dept Mol Biol, Umea, Sweden., 2015. Vol. 12, no 9, 985-997 p.
Keyword [en]
cell envelope stress, Listeria monocytogenes, proteomics, sRNA, transcriptomics
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-110203DOI: 10.1080/15476286.2015.1071011ISI: 000361473300012PubMedID: 26176322OAI: oai:DiVA.org:umu-110203DiVA: diva2:862063
Available from: 2015-10-20 Created: 2015-10-16 Last updated: 2015-10-20Bibliographically approved

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Johansson, Jörgen
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Department of Molecular Biology (Faculty of Medicine)
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RNA Biology
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