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Real-time 31P NMR investigation on the catalytic behavior of the enzyme Adenylate kinase in the matrix of a switchable ionic liquid
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry. Laboratory of Industrial Chemistry and Reaction Engineering, Johan Gadolin Process Chemistry Centre, Åbo Akademi University, Åbo-Turku, Finland.
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2015 (English)In: ChemSusChem, ISSN 1864-5631, E-ISSN 1864-564X, Vol. 8, no 2, 3764-3768 p.Article in journal (Refereed) Published
Abstract [en]

The integration of highly efficient enzymatic catalysis with the solvation properties of ionic liquids for an environmentally friendly and efficient use of raw materials such as wood requires fundamental knowledge about the influence of relevant ionic liquids on enzymes. Switchable ionic liquids (SIL) are promising candidates for implementation of enzymatic treatments of raw materials. One industrially interesting SIL is constituted by monoethanol amine (MEA) and 1,8-diazabicyclo-[5.4.0]-undec-7-ene (DBU) formed with sulfur dioxide (SO2) as the coupling media (DBU-SO2-MEASIL). It has the ability to solubilize the matrix of lignocellulosic biomass while leaving the cellulose backbone intact. Using a novel 31P  NMR-based real-time assay we show that this SIL is compatible with enzymatic catalysis because a model enzyme, adenylate kinase, retains its activity in up to at least 25 wt % of DBU-SO2-MEASIL. Thus this SIL appears suitable for, for example, enzymatic degradation of hemicellulose.

Place, publisher, year, edition, pages
John Wiley & Sons, 2015. Vol. 8, no 2, 3764-3768 p.
Keyword [en]
cellulose, enzymes, green chemistry, NMR spectroscopy, ionic liquids
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:umu:diva-110689DOI: 10.1002/cssc.201501104ISI: 000365752200003OAI: oai:DiVA.org:umu-110689DiVA: diva2:864247
Available from: 2015-10-26 Created: 2015-10-26 Last updated: 2015-12-29Bibliographically approved

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Rogne, PerSparrman, TobiasAnugwom, IkennaMikkola, Jyri-PekkaWolf-Watz, Magnus
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