Expression of an interferon-gamma-like substance in normal and transplanted rat heart tissue
1992 (English)In: J Heart Lung Transplant, Vol. 11, no 1 Pt 1, 142-6 p.Article in journal (Refereed) Published
The presence of interferon-gamma-like molecules has been reported not only in lymphocytes, but also in certain nerve cells and in normal skeletal muscle. We have studied the reactivity of the anti-interferon-gamma monoclonal antibody DB1 with frozen sections of normal and transplanted rat hearts. Cardiac grafts from PVG donor rats were transplanted to syngeneic PVG recipients or allogeneic Wistar/Kyoto recipient rats with the use of an accessory cervical heart transplantation technique. The allogeneic heart transplants were harvested 4 days and the syngeneic grafts 4 weeks after transplantation. In normal hearts there was a weak but distinct reactivity with the anti-interferon-gamma antibody in most muscle cells. In addition, some lymphocytes and the Purkinje fibers were positive. Hearts transplanted over an allogeneic barrier revealed that staining for interferon-gamma on muscle cells was substantially increased whereas no or only a moderate increase in the anti-interferon-gamma staining was seen in hearts transplanted to syngeneic recipients. These data indicate that interferon-gamma present in rat myocyte may be involved in the pathophysiology of graft rejection and also suggest that interferon-gamma may be of importance for the function of normal rat heart muscle cells.
Place, publisher, year, edition, pages
1992. Vol. 11, no 1 Pt 1, 142-6 p.
Animals, Antibodies, Monoclonal/immunology, Graft Rejection/*immunology, Heart Transplantation/*immunology, Interferon-gamma/*analysis, Male, Myocardium/*chemistry/immunology, Rats, Rats, Inbred Strains
IdentifiersURN: urn:nbn:se:umu:diva-110950ISBN: 1053-2498 (Print) 1053-2498 (Linking)OAI: oai:DiVA.org:umu-110950DiVA: diva2:865823
Wanders, A Wells, A F Larsson, E Tufveson, G Olsson, T Ljungdahl, A Klareskog, L eng Research Support, Non-U.S. Gov't 1992/01/01 J Heart Lung Transplant. 1992 Jan-Feb;11(1 Pt 1):142-6.2015-10-292015-10-292015-10-29