Sentinel node lymphocytes: tumour reactive lymphocytes identified intraoperatively for the use in immunotherapy of colon cancer
2006 (English)In: Br J Cancer, Vol. 94, no 10, 1478-84 p.Article in journal (Refereed) Published
The sentinel node is the first lymph node to receive lymphatic drainage from a tumour and is usually the first site of metastases. Today, the sentinel node is used for tumour staging. Here, we focus on its immunological role and investigate lymphocytic function in sentinel nodes, identified intraoperatively by peritumoural dye injection, from 15 patients with colon cancer. Tumour infiltrating lymphocytes, sentinel and nonsentinel lymph node cells and peripheral blood leukocytes were studied by flow cytometry, proliferation assays and interferon-gamma secretion after activation with autologous tumour homogenate. Whereas tumour-infiltrating lymphocytes were nonresponsive in the proliferation assays, lymphocytes from sentinel nodes proliferated dose dependently and secreted interferon-gamma upon stimulation with tumour homogenate. The responses were of varying magnitude and tended to be weaker in metastatic sentinel nodes. Sentinel node lymphocytes represents an enriched source of tumour reactive lymphocytes, and may be useful in future trials of adoptive immunotherapy.
Place, publisher, year, edition, pages
2006. Vol. 94, no 10, 1478-84 p.
Aged, Aged, 80 and over, CD4-Positive T-Lymphocytes/*immunology, CD8-Positive T-Lymphocytes/*immunology, Colonic Neoplasms/*immunology/pathology/surgery, Female, Flow Cytometry, Humans, Immunotherapy, Lymph Nodes/immunology, Lymphatic Metastasis, Lymphocyte Activation, Lymphocytes, Tumor-Infiltrating/*immunology, Male, Middle Aged, Neoplasm Staging, Sentinel Lymph Node Biopsy, Survival Rate
IdentifiersURN: urn:nbn:se:umu:diva-110922ISBN: 0007-0920 (Print) 0007-0920 (Linking)OAI: oai:DiVA.org:umu-110922DiVA: diva2:865862
Marits, P Karlsson, M Dahl, K Larsson, P Wanders, A Thorn, M Winqvist, O eng Research Support, Non-U.S. Gov't England 2006/04/28 09:00 Br J Cancer. 2006 May 22;94(10):1478-84.2015-10-292015-10-292015-10-29