Change search
ReferencesLink to record
Permanent link

Direct link
Cytokine gene expression: analysis using northern blotting, polymerase chain reaction and in situ hybridization
Show others and affiliations
1991 (English)In: Immunol Rev, Vol. 119, 163-79 p.Article in journal (Refereed) Published
Abstract [en]

We describe here the use of northern blotting, PCR and in situ hybridization for the analysis of cytokine gene expression. These techniques, each with their advantages and disadvantages, have been used to monitor cytokine gene expression in sites of immune reactivity and in the developing thymus. Whilst expression of a gene usually correlates well with protein production from that gene, this may not always be the case. The development of methods to analyze protein production in situ, for instance by immunohistochemistry, together with analysis of mRNA expression will allow us to begin to understand the role of cytokines within the immune system of the intact animal.

Place, publisher, year, edition, pages
1991. Vol. 119, 163-79 p.
Keyword [en]
Animals, Blotting, Northern, Cytokines/biosynthesis/*genetics, Gene Expression, Immune Tolerance/genetics, Nucleic Acid Hybridization, Polymerase Chain Reaction, RNA, Messenger/genetics/metabolism, Transplantation Immunology/genetics
National Category
Basic Medicine
URN: urn:nbn:se:umu:diva-110901ISBN: 0105-2896 (Print) 0105-2896 (Linking)OAI: diva2:865887

Dallman, M J Montgomery, R A Larsen, C P Wanders, A Wells, A F eng Research Support, Non-U.S. Gov't Review DENMARK 1991/02/01 Immunol Rev. 1991 Feb;119:163-79.

Available from: 2015-10-29 Created: 2015-10-29 Last updated: 2015-10-29

Open Access in DiVA

No full text

Other links

Search in DiVA

By author/editor
Wanders, A.
Basic Medicine

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 10 hits
ReferencesLink to record
Permanent link

Direct link