umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
High Sensitivity Method to Estimate Distribution of Hyaluronan Molecular Sizes in Small Biological Samples Using Gas-Phase Electrophoretic Mobility Molecular Analysis.
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Medicine. Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
Show others and affiliations
2015 (English)In: International Journal of Cell Biology, ISSN 1687-8876, E-ISSN 1687-8884, 938013Article in journal (Refereed) Published
Abstract [en]

Hyaluronan is a negatively charged polydisperse polysaccharide where both its size and tissue concentration play an important role in many physiological and pathological processes. The various functions of hyaluronan depend on its molecular size. Up to now, it has been difficult to study the role of hyaluronan in diseases with pathological changes in the extracellular matrix where availability is low or tissue samples are small. Difficulty to obtain large enough biopsies from human diseased tissue or tissue from animal models has also restricted the study of hyaluronan. In this paper, we demonstrate that gas-phase electrophoretic molecular mobility analyzer (GEMMA) can be used to estimate the distribution of hyaluronan molecular sizes in biological samples with a limited amount of hyaluronan. The low detection level of the GEMMA method allows for estimation of hyaluronan molecular sizes from different parts of small organs. Hence, the GEMMA method opens opportunity to attain a profile over the distribution of hyaluronan molecular sizes and estimate changes caused by disease or experimental conditions that has not been possible to obtain before.

Place, publisher, year, edition, pages
2015. 938013
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:umu:diva-111029DOI: 10.1155/2015/938013PubMedID: 26448761OAI: oai:DiVA.org:umu-111029DiVA: diva2:866196
Available from: 2015-11-02 Created: 2015-11-02 Last updated: 2017-12-01Bibliographically approved

Open Access in DiVA

fulltext(1250 kB)129 downloads
File information
File name FULLTEXT01.pdfFile size 1250 kBChecksum SHA-512
0d6311ac43597e65519ab3bd4a067647a86393b591e69d65d2cc5c89f6fb869248e85037f30ec77c72c705931a8ba17cd152ad066d616252c531546c45485b3c
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Do, LanMörner, StellanEngström-Laurent, AnnaLarsson, GöranHellman, Urban

Search in DiVA

By author/editor
Do, LanMörner, StellanEngström-Laurent, AnnaLarsson, GöranHellman, Urban
By organisation
MedicineDepartment of Medical Biochemistry and BiophysicsCardiology
In the same journal
International Journal of Cell Biology
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Search outside of DiVA

GoogleGoogle Scholar
Total: 129 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 119 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf