Hyaluronan stimulates keratinocyte migration and activates the transcription factor AP-1 in keratinocytes through the JNK pathway
2002 (English)In: Hyaluronan, Vol 1: Chemical, Biochemical and Biological Aspects / [ed] Kennedy JF, Phillips GO, Williams PA, Hascall VC, Great Britain: Woodhead Publishing Limited, 2002, Vol. 1, 551-556 p.Conference paper (Refereed)
Hyaluronan (HA) has been considered a passive extracellular matrix (ECM) polysaccharide, but recent studies have shown its importance in controlling many cell functions including motility, proliferation and adhesion, which imply signaling from ECM to cytosol. Hyaluronan is a major ECM component in stratified epithelia such as skin epidermis. We found that hyaluronan added to the growth medium of newly plated human skin keratinocytes increased cell migration in an in vitro wound-healing assay. Hyaluronan also increased the transcription factor AP-1, as determined by gel shift assays. The kinase signals that apperently led to the increased AP-1 level were associated with the activation of c-Jun, mainly via the JNK pathway as early as 10 min after the addition of hyaluronan, and with the minimum concentration of 10 ng/ml. ERK1 was also slightly activated, while p38 MAPkinase was not affected.
Place, publisher, year, edition, pages
Great Britain: Woodhead Publishing Limited, 2002. Vol. 1, 551-556 p.
Hyaluronan, AP-1, keratinocyte, Mitogen-activated protein kinase
Cell and Molecular Biology Biochemistry and Molecular Biology Dermatology and Venereal Diseases
Research subject Biochemistry; cellforskning; Dermatology and Venerology
IdentifiersURN: urn:nbn:se:umu:diva-111587DOI: 10.1533/9781845693121.551ISBN: 1855735709OAI: oai:DiVA.org:umu-111587DiVA: diva2:871934