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Genome-wide study identifies the regulatory glycosyltransferase genes networks and signaling pathways from Keshan disease
Key Laboratory of Environment and Genes Related to Diseases, Xi’an Jiaotong University, Ministry of Education, Xi’an, China; Clinical Laboratory, The First Hospital of Yulin City, Yulin, China.
Key Laboratory of Environment and Genes Related to Diseases, Xi’an Jiaotong University, Ministry of Education, Xi’an, China.
Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland. (Chondrogenic and Osteogenic Differentiation Group)ORCID iD: 0000-0002-1710-7715
Key Laboratory of Environment and Genes Related to Diseases, Xi’an Jiaotong University, Ministry of Education, Xi’an, China.
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2014 (English)In: Journal of Health Science, Vol. 2, no 4, 165-173 p.Article in journal (Refereed) Published
Abstract [en]

KD (Keshan disease) is an endemic cardiomyopathy occurring only in China. Its pathogenesis is unclear till now. In the study, gene expression profiles of the PBMC (peripheral blood mononuclear cell) derived respectively from KD patients and healthy in KD areas were compared. Total RNA was isolated, amplified, labeled and hybridized to Agilent 4 × 44 K Whole Human Genome Oligonucleotide Microarray. Significant canonical pathways were analyzed by IPA (ingenuity pathway analysis) to identify differently expressed genes and pathways involved in the cardiovascular system development and function. Quantitative RT-PCR was applied tofurther validate our microarray results. Eighty-three up-regulated (ratios ≥ 2.0) and nine down-regulated glycosyltransferase genes (ratios ≤ 0.5) in PBMC in KD patients were detected by significance analysis of microarrays. Two significant canonical pathways from glycosyltransferase gene expression profiles were screened by IPA. The results of qRT-PCR show that four up-regulated (BMP1/7/10 and FGF18) and one down-regulated (BMP2) genes are consistent with those in microarray experiment, confirming the validity of the microarray data. Based on the results of the study, it is suggested that bone morphogenetic proteins and fibroblast growth factors might play an important role in the pathogenesis of KD. This further helps us to understand the pathogenesis of KD, as well as dilated cardiomyopathy

Place, publisher, year, edition, pages
David Publishing Company, 2014. Vol. 2, no 4, 165-173 p.
Keyword [en]
eshan disease, glycosyltransferase gene, signaling pathway, bone morphogeneti c protein, fibroblast growth factor
National Category
Cell and Molecular Biology Cardiac and Cardiovascular Systems Biochemistry and Molecular Biology
Research subject
Biochemistry; Cardiology; cellforskning
Identifiers
URN: urn:nbn:se:umu:diva-111603OAI: oai:DiVA.org:umu-111603DiVA: diva2:872054
Available from: 2015-11-17 Created: 2015-11-17 Last updated: 2016-02-04

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Qu, Chengjuan
Cell and Molecular BiologyCardiac and Cardiovascular SystemsBiochemistry and Molecular Biology

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