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Profiling of embryonic nuclear vs. cellular RNA in Arabidopsis thaliana
Max Planck Institute for Developmental Biology, Department of Molecular Biology, Tübingen, Germany.ORCID iD: 0000-0002-0068-2967
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2015 (English)In: Genomics Data, ISSN 1025-6059, E-ISSN 2213-5960, Vol. 4, 96-98 p.Article in journal (Refereed) Published
Abstract [en]

In Arabidopsis, various cell type-specific whole-genome expression analyses have been conducted. However, the vast majority of these were performed with cellular RNA from root tissues or other easily accessible cell types [1]. Nuclear RNA was neglected for a long time as not being representative for transcriptomic studies. In recent years, however, there have been reports describing the validity of nuclear RNA for these types of studies [2] and [3]. Here we describe the generation, quality assessment and analysis of nuclear transcriptomic data from Arabidopsis embryos published by Slane et al. (2014) [4]. Comparison of nuclear with cellular gene expression demonstrated the usefulness of nuclear transcriptomics.

Place, publisher, year, edition, pages
Elsevier, 2015. Vol. 4, 96-98 p.
Keyword [en]
Arabidopsis thaliana, Gene expression, Microarray, Nuclear and cellular transcriptome, Pro-embryo and suspensor transcriptome
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URN: urn:nbn:se:umu:diva-111799DOI: 10.1016/j.gdata.2015.03.015ISI: 000362674500025PubMedID: 26484189OAI: diva2:873485
Available from: 2015-11-24 Created: 2015-11-24 Last updated: 2016-03-21Bibliographically approved

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Schmid, Markus
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