Probing S-state advancements and recombination pathways in photosystem II with a global fit program for flash-induced oxygen evolution pattern
2016 (English)In: Biochimica et Biophysica Acta - Bioenergetics, ISSN 0005-2728, E-ISSN 1879-2650, Vol. 1857, no 6, 848-859 p.Article in journal (Refereed) Published
The oxygen-evolving complex (OEC) in photosystem II catalyzes the oxidation of water to molecular oxygen. Four decades ago, measurements of flash-induced oxygen evolution have shown that the OEC steps through oxidation states S0, S1, S2, S3 and S4 before O2 is released and the S0 state is reformed. The light-induced transitions between these states involve misses and double hits. While it is widely accepted that the miss parameter is S state dependent and may be further modulated by the oxidation state of the acceptor side, the traditional way of analyzing each flash-induced oxygen evolution pattern (FIOP) individually did not allow using enough free parameters to thoroughly test this proposal. Furthermore, this approach does not allow assessing whether the presently known recombination processes in photosystem II fully explain all measured oxygen yields during Si state lifetime measurements. Here we present a global fit program that simultaneously fits all flash-induced oxygen yields of a standard FIOP (2 Hz flash frequency) and of 11–18 FIOPs each obtained while probing the S0, S2 and S3 state lifetimes in spinach thylakoids at neutral pH. This comprehensive data treatment demonstrates the presence of a very slow phase of S2 decay, in addition to the commonly discussed fast and slow reduction of S2 by YD and QB−, respectively. Our data support previous suggestions that the S0 → S1 and S1 → S2 transitions involve low or no misses, while high misses occur in the S2 → S3 or S3 → S0 transitions.
Place, publisher, year, edition, pages
2016. Vol. 1857, no 6, 848-859 p.
Photosynthesis, Photosystem 2 (PSII), Oxygen (O2) evolution, Water oxidation, Kok model, Flash induced oxygen evolution pattern (FIOP)
Research subject Physical Chemistry
IdentifiersURN: urn:nbn:se:umu:diva-111855DOI: 10.1016/j.bbabio.2016.03.013ISI: 000376700000024OAI: oai:DiVA.org:umu-111855DiVA: diva2:873739
Originally published in manuscript form with title Probing S-state advancements and recombination pathways in photosystem II with a global fit program for flash-induced oxygen evolution yields2015-11-242015-11-242016-07-26Bibliographically approved