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YscU/FlhB of Yersinia pseudotuberculosis Harbors a C-terminal Type III Secretion Signal
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
2015 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 290, no 43, 26282-26291 p.Article in journal (Refereed) Published
Abstract [en]

All type III secretion systems (T3SS) harbor a member of the YscU/FlhB family of proteins that is characterized by an auto-proteolytic process that occurs at a conserved cytoplasmic NPTH motif. We have previously demonstrated that YscU(CC), the C-terminal peptide generated by auto-proteolysis of Yersinia pseudotuberculosis YscU, is secreted by the T3SS when bacteria are grown in Ca2+ -depleted medium at 37 degrees C. Here, we investigated the secretion of this early T3S-substrate and showed that YscU(CC) encompasses a specific C-terminal T3S signal within the 15 last residues (U-15). U-15 promoted C-terminal secretion of reporter proteins like GST and YopE lacking its native secretion signal. Similar to the "classical" N-terminal secretion signal, U-15 interacted with the ATPase YscN. Although U-15 is critical for YscU(CC) secretion, deletion of the C-terminal secretion signal of YscU(CC) did neither affect Yop secretion nor Yop translocation. However, these deletions resulted in increased secretion of YscF, the needle subunit. Thus, these results suggest that YscU via its C-terminal secretion signal is involved in regulation of the YscF secretion.

Place, publisher, year, edition, pages
Umea Univ, Dept Mol Biol, Umea Ctr Microbial Res, SE-90187 Umea, Sweden. Umea Univ, Lab Mol Infect Med Sweden MIMS, Umea Ctr Microbial Res, SE-90187 Umea, Sweden., 2015. Vol. 290, no 43, 26282-26291 p.
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-111762DOI: 10.1074/jbc.M114.633677ISI: 000363527300044PubMedID: 26338709OAI: oai:DiVA.org:umu-111762DiVA: diva2:874089
Available from: 2015-11-25 Created: 2015-11-23 Last updated: 2015-11-25Bibliographically approved

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Login, Frederic H.Wolf-Watz, Hans
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Department of Molecular Biology (Faculty of Medicine)Umeå Centre for Microbial Research (UCMR)Molecular Infection Medicine Sweden (MIMS)
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Journal of Biological Chemistry
Microbiology in the medical area

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