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Relative and absolute reliability of measures of linoleic acid-derived oxylipins in human plasma
Umeå University, Faculty of Science and Technology, Department of Chemistry. Umeå University, Faculty of Medicine, Department of Pharmacology and Clinical Neuroscience, Pharmacology.
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Pulmonary Medicine.
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Pulmonary Medicine.
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Pulmonary Medicine.
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2015 (English)In: Prostaglandins & other lipid mediators, ISSN 1098-8823, E-ISSN 2212-196X, Vol. 121, no Part B, 227-233 p.Article in journal (Refereed) Published
Abstract [en]

Modern analytical techniques allow for the measurement of oxylipins derived from linoleic acid in biological samples. Most validatory work has concerned extraction techniques, repeated analysis of aliquots from the same biological sample, and the influence of external factors such as diet and heparin treatment upon their levels, whereas less is known about the relative and absolute reliability of measurements undertaken on different days. A cohort of nineteen healthy males were used, where samples were taken at the same time of day on two occasions, at least 7 days apart. Relative reliability was assessed using Lin's concordance correlation coefficients (CCC) and intraclass correlation coefficients (ICC). Absolute reliability was assessed by Bland-Altman analyses. Nine linoleic acid oxylipins were investigated. ICC and CCC values ranged from acceptable (0.56 [13-HODE]) to poor (near zero [9(10)- and 12(13)-EpOME]). Bland-Altman limits of agreement were in general quite wide, ranging from ±0.5 (12,13-DiHOME) to ±2 (9(10)-EpOME; log10 scale). It is concluded that relative reliability of linoleic acid-derived oxylipins varies between lipids with compounds such as the HODEs showing better relative reliability than compounds such as the EpOMEs. These differences should be kept in mind when designing and interpreting experiments correlating plasma levels of these lipids with factors such as age, body mass index, rating scales etc.

Place, publisher, year, edition, pages
Elsevier, 2015. Vol. 121, no Part B, 227-233 p.
Keyword [en]
Oxylipins, Arachidonic acid, HETE, Linoleic acid, HODE, DiHOME, Repeatability, Reliability, Bland–Altman analyses, Concordance correlation coefficients, Intraclass correlation coefficients, Human plasma
National Category
Chemical Sciences Biochemistry and Molecular Biology Cell Biology
Identifiers
URN: urn:nbn:se:umu:diva-112032DOI: 10.1016/j.prostaglandins.2015.10.003ISI: 000367636300011PubMedID: 26498702OAI: oai:DiVA.org:umu-112032DiVA: diva2:874971
Available from: 2015-11-30 Created: 2015-11-30 Last updated: 2016-05-16Bibliographically approved

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Gouveia-Figueira, SandraBosson, Jenny A.Unosson, JonBehndig, Annelie F.Nording, MalinFowler, Christopher
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Chemical SciencesBiochemistry and Molecular BiologyCell Biology

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