umu.sePublications
Change search
ReferencesLink to record
Permanent link

Direct link
Regulation of replication and maintenance functions of broad host-range plasmid RK2.
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology).ORCID iD: 0000-0002-7349-1678
Show others and affiliations
1985 (English)In: Basic life sciences, ISSN 0090-5542, Vol. 30Article in journal (Refereed) Published
Abstract [en]

Replication of broad host-range plasmid RK2 depends on a cisacting vegatative replication origin oriVRK2 and the polypeptide product(s) of the trans-acting gene trfA as well as on host-specified products. The trfA gene is the second cistron in a polycistronic unit whose first cistron may be kilD, one of 4 known RK2-specified kil loci (kilA, B, C, and D) which are inhibitory for bacterial host or plasmid vector in the absence of kor functions which suppress in trans the effect of their respective kil genes. Transcription of the operon containing trfA is negatively regulated by the products of both the trfB locus (alias korD and korA) and korB. The loci, trfB and korB, are expressed from a single transcriptional unit which we propose to be negatively autoregulated by the products of both loci, although an additional, weaker and unregulated transcript may also express korB. While deletions in the oriVRK2 region have indicated the presence of copy number control elements adjacent to and possibly overlapping with the minimal oriVRK2 segment, the overriding control of copy number seems to reside in the trfB and korB loci which in conjunction appear to reduce expression of the trfA gene to levels limiting for replication. Coregulation of trfA with kil genes may indicate that kil genes play a role in plasmid maintenance other than replication.

Place, publisher, year, edition, pages
1985. Vol. 30
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:umu:diva-112359PubMedID: 2990413OAI: oai:DiVA.org:umu-112359DiVA: diva2:877247
Available from: 2015-12-06 Created: 2015-12-06 Last updated: 2015-12-06

Open Access in DiVA

No full text

PubMed

Search in DiVA

By author/editor
Shingler, V
By organisation
Department of Molecular Biology (Faculty of Science and Technology)
Natural Sciences

Search outside of DiVA

GoogleGoogle ScholarTotal: 1 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 9 hits
ReferencesLink to record
Permanent link

Direct link