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The trfA and trfB promoter regions of broad host range plasmid RK2 share common potential regulatory sequences.
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology).ORCID iD: 0000-0002-7349-1678
1984 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 12, no 8Article in journal (Refereed) Published
Abstract [en]

The positions of the trfA and trfB promoters of broad host range IncP plasmid RK2 (identical to RP1, RP4, R68 and R18 ) were identified by RNA polymerase protection studies, and the nucleotide sequences of the promoter regions determined. A mutation within the trfA promoter sequence is associated with loss of kilD activity. In addition a probable promoter region for the kilB locus was identified. The three promoter regions share common palindromic sequences which may serve as sites for the coordinate regulation of replication and kil functions.

Place, publisher, year, edition, pages
1984. Vol. 12, no 8
National Category
Natural Sciences
URN: urn:nbn:se:umu:diva-112357PubMedID: 6328430OAI: diva2:877250
Available from: 2015-12-06 Created: 2015-12-06 Last updated: 2015-12-06

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